A convenient workflow to spot photosensitizers revealed photo-activity in basidiomycetes

Photodynamic therapy (PDT) is an alternative approach for the treatment of neoplastic diseases employing photosensitizers activated by light. In order to discover new natural photosensitizers, a convenient workflow was established. To validate the workflow, fungi were selected, because we hypothesiz...

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Bibliographic Details
Published in:RSC advances 2019-02, Vol.9 (8), p.4545-4552
Main Authors: Siewert, Bianka, Vrabl, Pamela, Hammerle, Fabian, Bingger, Isabella, Stuppner, Hermann
Format: Article
Language:English
Subjects:
Chemistry
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Summary:Photodynamic therapy (PDT) is an alternative approach for the treatment of neoplastic diseases employing photosensitizers activated by light. In order to discover new natural photosensitizers, a convenient workflow was established. To validate the workflow, fungi were selected, because we hypothesized that fruiting bodies and mycelia are an overlooked source. The results proved the hypothesis, as exorbitant high photo-cytotoxicity values were detected. For example, the acetone extract of was characterized by an EC of 1 μg mL against cells of a lung cancer cell-line (A549). In sum, a low-cost workflow for the detection and biological evaluation of photosensitizers is presented and discussed. Furthermore, this paper provides the first experimental evidence for phototoxic metabolites in basidiomycetes. This hints towards a new assignable function of fungal pigments, photochemical defense.
ISSN:2046-2069
2046-2069
DOI:10.1039/c8ra10181g