A novel approach for increasing transformation efficiency in E. coli DH5α cells using silver nanoparticles

The present study is the first report on the application of silver nanoparticles for efficient bacterial transformation. EC50 value of 100 nm silver nanoparticles against E. coli DH5α cells was recorded as 4.49 mg L −1 in toxicity assay. Competency induction in E. coli DH5α cells by treatment with 1...

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Bibliographic Details
Published in:3 Biotech 2019-03, Vol.9 (3), p.113-113, Article 113
Main Authors: Nagamani, Gorantla, Alex, Swapna, Soni, K. B., Anith, K. N., Viji, M. M., Kiran, A. G.
Format: Article
Language:English
Subjects:
Agriculture
Bioinformatics
Biomaterials
Biotechnology
Calcium chloride
Cancer Research
Chemistry
Chemistry and Materials Science
Deoxyribonucleic acid
DNA
E coli
Gold
Heat shock
Nanoparticles
Original
Original Article
Silver
Stem Cells
Toxicity
Transformations
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Summary:The present study is the first report on the application of silver nanoparticles for efficient bacterial transformation. EC50 value of 100 nm silver nanoparticles against E. coli DH5α cells was recorded as 4.49 mg L −1 in toxicity assay. Competency induction in E. coli DH5α cells by treatment with 100 nm silver nanoparticles at a concentration of 1 mg L −1 for 60 min and transformation using three plasmid vectors of different sizes, viz. pUC18, pBR322 and pCAMBIA resulted in tenfold increase in the bacterial transformation efficiency, i.e. 8.3 × 10 4 , 8.0 × 10 4 and 7.9 × 10 4  cfu ng −1 of DNA, respectively, even without heat shock compared to the conventional chemical method using 0.1 M calcium chloride (2.3 × 10 3  cfu ng −1 of DNA).
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-019-1640-9