Microbial Colonization Coordinates the Pathogenesis of a Klebsiella pneumoniae Infant Isolate

• Published in: Scientific reports 2019-03, Vol.9 (1), p.3380-3380, Article 3380
• Main Authors: Pope, Jillian L., Yang, Ye, Newsome, Rachel C., Sun, Wei, Sun, Xiaolun, Ukhanova, Maria, Neu, Josef, Issa, Jean-Pierre, Mai, Volker, Jobin, Christian
• Format: Article
• Language: English
• Subjects: 13/106; 13/21; 13/31; 38/77; 631/326; 631/67; 692/4020; 82/51; Animals; Cell cycle; Cell Cycle Checkpoints; Colonization; Colony Count, Microbial; Colorectal carcinoma; Colorectal Neoplasms - microbiology; DNA Damage; Epithelial cells; Gene regulation; Gnotobiotic; Humanities and Social Sciences; Humans; Infant, Newborn; Infant, Premature; Inflammation; Inflammation - genetics; Interleukin 1; Interleukin 10; Intestine; Intestines - microbiology; Intestines - pathology; Klebsiella pneumoniae - isolation & purification; Klebsiella pneumoniae - pathogenicity; Macrophages; Mice; Microbiota; multidisciplinary; Neonates; Pathogenicity; Science; Science (multidisciplinary); Splenocytes; Tumors
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-39887-8

Enterobacteriaceae are among the first colonizers of neonate intestine. Members of this family, such as Escherichia and Klebsiella , are considered pathobionts and as such are capable of inducing local and systemic disease under specific colonization circumstances. Interplay between developing microbiota and pathogenic function of pathobionts are poorly understood. In this study, we investigate the functional interaction between various colonization patterns on an early colonizer, K . pneumoniae . K . pneumoniae 51-5 was isolated from stool of a healthy, premature infant, and found to contain the genotoxin island pks associated with development of colorectal cancer. Using intestinal epithelial cells, macrophages, and primary splenocytes, we demonstrate K . pneumoniae 51-5 upregulates expression of proinflammatory genes in vitro . Gnotobiotic experiments in Il10 −/− mice demonstrate the neonate isolate induces intestinal inflammation in vivo , with increased expression of proinflammatory genes. Regulation of microbiota assembly revealed K . pneumoniae 51-5 accelerates onset of inflammation in Il10 −/− mice, most significantly when microbiota is naturally acquired. Furthermore, K . pneumoniae 51-5 induces DNA damage and cell cycle arrest. Interestingly, K . pneumoniae 51-5 induced tumors in Apc Min /+ ; Il10 −/− mice was not significantly affected by absence of colibactin activating enzyme, ClbP. These findings demonstrate pathogenicity of infant K . pneumoniae isolate is sensitive to microbial colonization status.