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Selection and Validation of Reference Genes for Gene Expression Studies by RT-PCR in Dalbergia odorifera
Perennial tree Dalbergia odorifera T. Chen could form the precious heartwood used to produce chinese traditional medicine, rosewood furniture and fragrances. However the formation of heartwood is time-consuming and low efficient, leading to the severe destruction of its wild resources. Thus, it is u...
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| Published in: | Scientific reports 2019-03, Vol.9 (1), p.3341-3341, Article 3341 |
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| description | Perennial tree
Dalbergia odorifera
T. Chen could form the precious heartwood used to produce chinese traditional medicine, rosewood furniture and fragrances. However the formation of heartwood is time-consuming and low efficient, leading to the severe destruction of its wild resources. Thus, it is urgent to study the molecular mechanism of heartwood formation in
D
.
odorifera
. But till now, there is no report about the reference gene selection in this species. In this study, the expression stability of nine candidate reference genes were evaluated across different tissues and stems treated by wound and chemical stimulators. Four algorithms were applied to obtain the robust genes. The results support
HIS2
,
GAPDH
, and
CYP
to be the most stable reference genes in samples under different wound treatments while
DNAj
was the least stable. In different tissues,
HIS2
,
UBQ
, and
RPL
were the most stable reference genes while
DNAj
was the least stable. The selected reference genes were validated through the normalization of the qRT-PCR data of six heartwood related genes in terpene biosynthesis pathway and ethylene signal pathway. The results showed that their expression levels were accurate when they were normalized by the most stable reference gene
HIS2
, or by the combination of the two or three most stable reference genes. These results demonstrated that these selected reference genes are reliable. |
| doi_str_mv | 10.1038/s41598-019-39088-3 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6399326</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2187937038</sourcerecordid><originalsourceid>FETCH-LOGICAL-c511t-6df94d4e5f227d5aea86c4d49d7d803c59ced6715c1efeb8a614b321f597f39b3</originalsourceid><addsrcrecordid>eNp9kU1v1DAQhi0EolXpH-CALHHhEvBHHNsXJLS0BakS1bZwtRx7snWVtRc7Qe2_x7tbSukBXzzjeeb1jF6EXlPynhKuPpSWCq0aQnXDNVGq4c_QISOtaBhn7Pmj-AAdl3JD6hFMt1S_RAecKM6Fkofo-hJGcFNIEdvo8Q87Bm93aRrwEgbIEB3gM4hQ8JDyLsInt5sMpWyxy2n2odb6O7y8ai4WSxwi_mzHHvIqWJx8yqGq2FfoxWDHAsf39xH6fnpytfjSnH87-7r4dN44QenUdH7QrW9BDIxJLyxY1bn6oL30inAntAPfSSocrcP1yna07Tmjg9By4LrnR-jjXncz92vwDuKU7Wg2OaxtvjPJBvNvJYZrs0q_TMe15qyrAu_uBXL6OUOZzDoUB-NoI6S5GEaVYkTqTlb07RP0Js051vW2lNRcVqcqxfaUy6mUDMPDMJSYrZdm76WpXpqdl4bXpjeP13ho-eNcBfgeKLUUV5D__v0f2d-Ggqqi</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2187937038</pqid></control><display><type>article</type><title>Selection and Validation of Reference Genes for Gene Expression Studies by RT-PCR in Dalbergia odorifera</title><source>PubMed Central Free</source><source>Publicly Available Content Database</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature - nature.com Journals - Fully Open Access</source><creator>Meng, Hui ; Yang, Yun ; Gao, Zhi-Hui ; Wei, Jian-He</creator><creatorcontrib>Meng, Hui ; Yang, Yun ; Gao, Zhi-Hui ; Wei, Jian-He</creatorcontrib><description>Perennial tree
Dalbergia odorifera
T. Chen could form the precious heartwood used to produce chinese traditional medicine, rosewood furniture and fragrances. However the formation of heartwood is time-consuming and low efficient, leading to the severe destruction of its wild resources. Thus, it is urgent to study the molecular mechanism of heartwood formation in
D
.
odorifera
. But till now, there is no report about the reference gene selection in this species. In this study, the expression stability of nine candidate reference genes were evaluated across different tissues and stems treated by wound and chemical stimulators. Four algorithms were applied to obtain the robust genes. The results support
HIS2
,
GAPDH
, and
CYP
to be the most stable reference genes in samples under different wound treatments while
DNAj
was the least stable. In different tissues,
HIS2
,
UBQ
, and
RPL
were the most stable reference genes while
DNAj
was the least stable. The selected reference genes were validated through the normalization of the qRT-PCR data of six heartwood related genes in terpene biosynthesis pathway and ethylene signal pathway. The results showed that their expression levels were accurate when they were normalized by the most stable reference gene
HIS2
, or by the combination of the two or three most stable reference genes. These results demonstrated that these selected reference genes are reliable.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-39088-3</identifier><identifier>PMID: 30833587</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/39 ; 38/77 ; 631/208/199 ; 631/449/1659 ; 631/449/2661/1880 ; Biosynthesis ; Dalbergia odorifera ; Gene expression ; Glyceraldehyde-3-phosphate dehydrogenase ; Humanities and Social Sciences ; multidisciplinary ; Polymerase chain reaction ; Science ; Science (multidisciplinary) ; Wounds</subject><ispartof>Scientific reports, 2019-03, Vol.9 (1), p.3341-3341, Article 3341</ispartof><rights>The Author(s) 2019</rights><rights>This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-6df94d4e5f227d5aea86c4d49d7d803c59ced6715c1efeb8a614b321f597f39b3</citedby><cites>FETCH-LOGICAL-c511t-6df94d4e5f227d5aea86c4d49d7d803c59ced6715c1efeb8a614b321f597f39b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2187937038/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2187937038?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30833587$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Meng, Hui</creatorcontrib><creatorcontrib>Yang, Yun</creatorcontrib><creatorcontrib>Gao, Zhi-Hui</creatorcontrib><creatorcontrib>Wei, Jian-He</creatorcontrib><title>Selection and Validation of Reference Genes for Gene Expression Studies by RT-PCR in Dalbergia odorifera</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Perennial tree
Dalbergia odorifera
T. Chen could form the precious heartwood used to produce chinese traditional medicine, rosewood furniture and fragrances. However the formation of heartwood is time-consuming and low efficient, leading to the severe destruction of its wild resources. Thus, it is urgent to study the molecular mechanism of heartwood formation in
D
.
odorifera
. But till now, there is no report about the reference gene selection in this species. In this study, the expression stability of nine candidate reference genes were evaluated across different tissues and stems treated by wound and chemical stimulators. Four algorithms were applied to obtain the robust genes. The results support
HIS2
,
GAPDH
, and
CYP
to be the most stable reference genes in samples under different wound treatments while
DNAj
was the least stable. In different tissues,
HIS2
,
UBQ
, and
RPL
were the most stable reference genes while
DNAj
was the least stable. The selected reference genes were validated through the normalization of the qRT-PCR data of six heartwood related genes in terpene biosynthesis pathway and ethylene signal pathway. The results showed that their expression levels were accurate when they were normalized by the most stable reference gene
HIS2
, or by the combination of the two or three most stable reference genes. These results demonstrated that these selected reference genes are reliable.</description><subject>38/39</subject><subject>38/77</subject><subject>631/208/199</subject><subject>631/449/1659</subject><subject>631/449/2661/1880</subject><subject>Biosynthesis</subject><subject>Dalbergia odorifera</subject><subject>Gene expression</subject><subject>Glyceraldehyde-3-phosphate dehydrogenase</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Polymerase chain reaction</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Wounds</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNp9kU1v1DAQhi0EolXpH-CALHHhEvBHHNsXJLS0BakS1bZwtRx7snWVtRc7Qe2_x7tbSukBXzzjeeb1jF6EXlPynhKuPpSWCq0aQnXDNVGq4c_QISOtaBhn7Pmj-AAdl3JD6hFMt1S_RAecKM6Fkofo-hJGcFNIEdvo8Q87Bm93aRrwEgbIEB3gM4hQ8JDyLsInt5sMpWyxy2n2odb6O7y8ai4WSxwi_mzHHvIqWJx8yqGq2FfoxWDHAsf39xH6fnpytfjSnH87-7r4dN44QenUdH7QrW9BDIxJLyxY1bn6oL30inAntAPfSSocrcP1yna07Tmjg9By4LrnR-jjXncz92vwDuKU7Wg2OaxtvjPJBvNvJYZrs0q_TMe15qyrAu_uBXL6OUOZzDoUB-NoI6S5GEaVYkTqTlb07RP0Js051vW2lNRcVqcqxfaUy6mUDMPDMJSYrZdm76WpXpqdl4bXpjeP13ho-eNcBfgeKLUUV5D__v0f2d-Ggqqi</recordid><startdate>20190304</startdate><enddate>20190304</enddate><creator>Meng, Hui</creator><creator>Yang, Yun</creator><creator>Gao, Zhi-Hui</creator><creator>Wei, Jian-He</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190304</creationdate><title>Selection and Validation of Reference Genes for Gene Expression Studies by RT-PCR in Dalbergia odorifera</title><author>Meng, Hui ; Yang, Yun ; Gao, Zhi-Hui ; Wei, Jian-He</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-6df94d4e5f227d5aea86c4d49d7d803c59ced6715c1efeb8a614b321f597f39b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>38/39</topic><topic>38/77</topic><topic>631/208/199</topic><topic>631/449/1659</topic><topic>631/449/2661/1880</topic><topic>Biosynthesis</topic><topic>Dalbergia odorifera</topic><topic>Gene expression</topic><topic>Glyceraldehyde-3-phosphate dehydrogenase</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Polymerase chain reaction</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Wounds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meng, Hui</creatorcontrib><creatorcontrib>Yang, Yun</creatorcontrib><creatorcontrib>Gao, Zhi-Hui</creatorcontrib><creatorcontrib>Wei, Jian-He</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meng, Hui</au><au>Yang, Yun</au><au>Gao, Zhi-Hui</au><au>Wei, Jian-He</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selection and Validation of Reference Genes for Gene Expression Studies by RT-PCR in Dalbergia odorifera</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2019-03-04</date><risdate>2019</risdate><volume>9</volume><issue>1</issue><spage>3341</spage><epage>3341</epage><pages>3341-3341</pages><artnum>3341</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Perennial tree
Dalbergia odorifera
T. Chen could form the precious heartwood used to produce chinese traditional medicine, rosewood furniture and fragrances. However the formation of heartwood is time-consuming and low efficient, leading to the severe destruction of its wild resources. Thus, it is urgent to study the molecular mechanism of heartwood formation in
D
.
odorifera
. But till now, there is no report about the reference gene selection in this species. In this study, the expression stability of nine candidate reference genes were evaluated across different tissues and stems treated by wound and chemical stimulators. Four algorithms were applied to obtain the robust genes. The results support
HIS2
,
GAPDH
, and
CYP
to be the most stable reference genes in samples under different wound treatments while
DNAj
was the least stable. In different tissues,
HIS2
,
UBQ
, and
RPL
were the most stable reference genes while
DNAj
was the least stable. The selected reference genes were validated through the normalization of the qRT-PCR data of six heartwood related genes in terpene biosynthesis pathway and ethylene signal pathway. The results showed that their expression levels were accurate when they were normalized by the most stable reference gene
HIS2
, or by the combination of the two or three most stable reference genes. These results demonstrated that these selected reference genes are reliable.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>30833587</pmid><doi>10.1038/s41598-019-39088-3</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Scientific reports, 2019-03, Vol.9 (1), p.3341-3341, Article 3341 |
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| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6399326 |
| source | PubMed Central Free; Publicly Available Content Database; Free Full-Text Journals in Chemistry; Springer Nature - nature.com Journals - Fully Open Access |
| subjects | 38/39 38/77 631/208/199 631/449/1659 631/449/2661/1880 Biosynthesis Dalbergia odorifera Gene expression Glyceraldehyde-3-phosphate dehydrogenase Humanities and Social Sciences multidisciplinary Polymerase chain reaction Science Science (multidisciplinary) Wounds |
| title | Selection and Validation of Reference Genes for Gene Expression Studies by RT-PCR in Dalbergia odorifera |
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