Solution Structure, Self-Assembly, and Membrane Interactions of the Matrix Protein from Newcastle Disease Virus at Neutral and Acidic pH

• Published in: Journal of virology 2019-03, Vol.93 (6)
• Main Authors: Shtykova, E V, Petoukhov, M V, Dadinova, L A, Fedorova, N V, Tashkin, V Yu, Timofeeva, T A, Ksenofontov, A L, Loshkarev, N A, Baratova, L A, Jeffries, C M, Svergun, D I, Batishchev, O V
• Format: Article
• Language: English
• Subjects: Animals; Cell Membrane - metabolism; Cell Membrane - virology; Chickens - virology; Endocytosis - physiology; Hydrogen-Ion Concentration; Lipid Bilayers - metabolism; Newcastle Disease - metabolism; Newcastle Disease - virology; Newcastle disease virus - metabolism; Newcastle disease virus - physiology; Structure and Assembly; Viral Matrix Proteins - metabolism; Virus Assembly - physiology; Virus Internalization
• ISSN: 0022-538X; 1098-5514; 1098-5514
• DOI: 10.1128/JVI.01450-18

Newcastle disease virus (NDV) is an enveloped paramyxovirus. The matrix protein of the virus (M-NDV) has an innate propensity to produce virus-like particles budding from the plasma membrane of the expressing cell without recruiting other viral proteins. The virus predominantly infects the host cell via fusion with the host plasma membrane or, alternatively, can use receptor-mediated endocytic pathways. The question arises as to what are the mechanisms supporting such diversity, especially concerning the assembling and membrane binding properties of the virus protein scaffold under both neutral and acidic pH conditions. Here, we suggest a novel method of M-NDV isolation in physiological ionic strength and employ a combination of small-angle X-ray scattering, atomic force microscopy with complementary structural techniques, and membrane interaction measurements to characterize the solution behavior/structure of the protein as well as its binding to lipid membranes at pH 4.0 and pH 7.0. We demonstrate that the minimal structural unit of the protein in solution is a dimer that spontaneously assembles in a neutral milieu into hollow helical oligomers by repeating the protein tetramers. Acidic pH conditions decrease the protein oligomerization state to the individual dimers, tetramers, and octamers without changing the density of the protein layer and lipid membrane affinity, thus indicating that the endocytic pathway is a possible facilitator of NDV entry into a host cell through enhanced scaffold disintegration. The matrix protein of the Newcastle disease virus (NDV) is one of the most abundant viral proteins that regulates the formation of progeny virions. NDV is an avian pathogen that impacts the economics of bird husbandry due to its resulting morbidity and high mortality rates. Moreover, it belongs to the subfamily of the family of that include dangerous representatives such as respiratory syncytial virus, human parainfluenza virus, and measles virus. Here, we investigate the solution structure and membrane binding properties of this protein at both acidic and neutral pH to distinguish between possible virus entry pathways and propose a mechanism of assembly of the viral matrix scaffold. This work is fundamental for understanding the mechanisms of viral entry as well as to inform subsequent proposals for the possible use of the virus as an adequate template for future drug or vaccine delivery.