Effect of 6-Month Vitamin D Supplementation on Plasma Matrix Gla Protein in Older Adults

• Published in: Nutrients 2019-01, Vol.11 (2), p.231
• Main Authors: van Ballegooijen, Adriana J, Beulens, Joline W J, Schurgers, Leon J, de Koning, Elisa J, Lips, Paul, van Schoor, Natasja M, Vervloet, Marc G
• Format: Article
• Language: English
• Subjects: Aged; Biomedical materials; Calciferol; Calcification; Calcification (ectopic); Calcium-Binding Proteins - blood; Calcium-Binding Proteins - metabolism; Cholecalciferol - administration & dosage; Cholecalciferol - pharmacology; Double-Blind Method; Extracellular Matrix Proteins - blood; Extracellular Matrix Proteins - metabolism; Female; Gene Expression Regulation - drug effects; Humans; Male; Matrix Gla Protein; Middle Aged; Older people; Osteocalcin; Phylloquinone; Secondary analysis; Studies; Systematic review; Vitamin D; Vitamin D3; Vitamin K; Womens health
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu11020231

Vitamin D supplementation has been widely promoted to restore 25-hydroxyvitamin D concentrations; however, experimental evidence suggests a nutrient interaction with vitamin K. We assessed the effects of 1200 IU vitamin D₃ per day versus placebo for six months on vitamin K status in a randomized, double-blind, placebo-controlled trial with participants aged 60⁻80 years with depressive symptoms and ≥1 functional limitation for a secondary analysis. Stored baseline and six-month follow-up blood samples were available for 131 participants ( = 65 placebo vs. = 66 vitamin D supplementation). We measured dephosphorylated uncarboxylated matrix gla protein (MGP) (dp-ucMGP) concentrations-a marker of vitamin K deficiency. Mean age was 68 years, and 89 participants (68%) were women. Vitamin K antagonists were used by 16 participants and multivitamin supplements by 50 participants. No differences in change between intervention and placebo were found (-38.5 ± 389 vs. 4.5 ± 127 (pmol/L), = 0.562). When excluding vitamin K antagonist users and multivitamin users, dp-ucMGP at follow-up was significantly higher in the vitamin D group ( = 40) compared to placebo ( = 30), with a difference of 92.8 (5.7, 180) pmol/L, adjusting for baseline dp-ucMGP and sex. In conclusion, vitamin D supplementation for six months did not affect vitamin K status; however, among participants without vitamin K antagonist or multivitamin use, vitamin D supplementation influenced dp-ucMGP concentrations.