Comparative effects of granulocyte-colony stimulating factor and colistin-alone or in combination on burn wound healing in Acinetobacter baumannii infected mice

• Published in: Iranian journal of microbiology 2018-12, Vol.10 (6), p.371-377
• Main Authors: Moghadam, Somayeh Soleymanzadeh, Aghmiyuni, Zeinab Fagheei, Zaheri, Hassan, Arianpour, Nastaran, Danaeifard, Mohammad Reza, Roham, Maryam, Momeni, Mahnoush
• Format: Article
• Language: English
• Subjects: Original
• ISSN: 2008-3289; 2008-4447

Burn wounds are one of the most important health problems all over the world because infection after burn can delay wound healing. Treating burn wounds with granulocyte-colony stimulating factor (G-CSF) is known to improve healing of injured tissue. In addition, colistin is prescribed as an effective treatment. The aim of this study was to evaluate the effect of G-CSF and colistin alone or in combination with G-CSF on wound healing of ( ) infected burns. This study was performed between January 2016 and April 2018. Burn wounds were experimentally induced in 36 mice. The wounds were inoculated with . In a 7-day period, burn wounds in each group were daily treated with subcutaneous injections (0.1 ml) of saline, G-CSF, colistin, and G-CSF plus colistin. After killing the animals, the size of the wound, number of leukocytes in the skin and microbial growth were evaluated. A value of p ≤ 0.05 was considered statistically significant. Wound healing in the G-CSF plus colistin group was significantly higher than the control group and the G-CSF group (P = 0.023 and P = 0.033, respectively). In G-CSF+colistin group, the number of leukocytes was higher than the control group considerably (P = 0.007). On the 7 day of treatment, number of positive bacterial cultures in the colistin and the G-CSF plus colistin groups was lower than other groups with a significant difference. Concurrent consumption of G-CSF and antibiotics can control burn infection and enhance the immune system towards wound healing.