Sortase-Mediated Ligation of Purely Artificial Building Blocks

Sortase A (SrtA) from has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene gly...

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Bibliographic Details
Published in:Polymers 2018-02, Vol.10 (2), p.151
Main Authors: Dai, Xiaolin, Mate, Diana M, Glebe, Ulrich, Mirzaei Garakani, Tayebeh, Körner, Andrea, Schwaneberg, Ulrich, Böker, Alexander
Format: Article
Language:English
Subjects:
Acrylamide
Buildings
Catalysis
Chemical synthesis
Electron microscopy
Ionization
Mass spectrometry
Nanoparticles
Photon correlation spectroscopy
Polyethylene glycol
Polymers
Reaction products
Silicon dioxide
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Summary:Sortase A (SrtA) from has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly( -isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP⁻polymer, NP⁻NP, and polymer⁻polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction-the conserved peptide LPETG-and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in cases where their synthesis by established chemical methods is complicated.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym10020151