Spicatoside A derived from Liriope platyphylla root ethanol extract inhibits hepatitis E virus genotype 3 replication in vitro

Hepatitis E virus (HEV) is the causative agent of hepatitis E in humans worldwide. Although hepatitis E is self-limiting without chronic infection development, HEV infection often leads to severe liver diseases causing high mortality in pregnant women in addition to chronic hepatitis and cirrhosis i...

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Bibliographic Details
Published in:Scientific reports 2019-03, Vol.9 (1), p.4397-4397, Article 4397
Main Authors: Park, Gayoung, Parveen, Amna, Kim, Jung-Eun, Cho, Kyo Hee, Kim, Sun Yeou, Park, Bang Ju, Song, Yoon-Jae
Format: Article
Language:English
Subjects:
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A549 Cells
Acetic acid
Animals
Antiviral Agents - chemistry
Antiviral Agents - pharmacology
Cell Line, Tumor
Chronic infection
Cirrhosis
Column chromatography
Ethanol
Ethanol - chemistry
Ethyl acetate
Fractionation
Genotype
Genotype & phenotype
Genotypes
Hepatitis
Hepatitis E virus - drug effects
Hepatitis E virus - pathogenicity
Humanities and Social Sciences
Humans
Liriope
Liriope Plant - chemistry
Liver cirrhosis
Liver diseases
multidisciplinary
Plant Extracts - chemistry
Plant Extracts - pharmacology
Replication
Saponins - chemistry
Saponins - pharmacology
Science
Science (multidisciplinary)
Virus Replication - drug effects
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Summary:Hepatitis E virus (HEV) is the causative agent of hepatitis E in humans worldwide. Although hepatitis E is self-limiting without chronic infection development, HEV infection often leads to severe liver diseases causing high mortality in pregnant women in addition to chronic hepatitis and cirrhosis in immunosuppressed patients. In this study, we investigated the effect of a Liriope platyphylla ethanol extract (LPE) on HEV replication. Interestingly, LPE suppressed replication of the genotype 3 HEV replicon. Sequential solvent fractionation revealed that the ethyl acetate (EA) fraction of LPE exerts the most potent inhibitory effects. With the aid of activity-guided fractionation and multi-step column chromatography, spicatoside A was subsequently isolated in the EA fraction of LPE and specifically shown to exert inhibitory effects on replication of the genotype 3 HEV replicon. In addition, spicatoside A interfered with replication of the HEV genotype 3 strain 47832c and expression of HEV ORF2 capsid proteins. Our findings clearly support the potential utility of spicatoside A as an effective anti-HEV agent.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-39488-5