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Warthin-Starry Staining for the Detection of Helicobacter pylori in Gastric Biopsies
Warthin-Starry (WS) staining is an ancillary stain used in the detection of , spirochaete and other microorganisms in tissue sections. The present study aimed to determine the validity of WS stain in the confirmation of diagnosis in gastric biopsies in comparison with anti immunohistochemistry (IHC)...
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| Published in: | The Malaysian journal of medical sciences 2018-07, Vol.25 (4), p.92-99 |
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| Main Authors: | , , , , |
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| container_end_page | 99 |
| container_issue | 4 |
| container_start_page | 92 |
| container_title | The Malaysian journal of medical sciences |
| container_volume | 25 |
| creator | Farouk, Wirda Indah Hassan, Nur Hidayah Ismail, Teh Rasyidah Daud, Intan Sufinaz Mohammed, Fazarina |
| description | Warthin-Starry (WS) staining is an ancillary stain used in the detection of
, spirochaete and other microorganisms in tissue sections. The present study aimed to determine the validity of WS stain in the confirmation of
diagnosis in gastric biopsies in comparison with anti
immunohistochemistry (IHC) staining.
This study involved 104 cases of gastric biopsies that were previously subjected to WS staining. All cases involved retrieval of formalin-fixed paraffin-embedded (FFPE) gastric biopsies that were re-cut, subjected to anti
IHC staining and reviewed blindly by a pathologist. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of WS as compared to IHC were calculated.
In this study, WS stain was less sensitive in detecting
. The sensitivity, specificity, PPV and NPV for WS stain were 50.0%, 92.4%, 79.2% and 76.3%, respectively.
The sensitivity of WS stain in the histopathology laboratory was lower than that described previously. Several external factors that might influence the results were identified. However, sufficient information on patients' history of treatment and medication would be required for the diagnosis or confirmation of the presence of
in gastric biopsies by WS staining. |
| doi_str_mv | 10.21315/mjms2018.25.4.9 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6422537</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2198560538</sourcerecordid><originalsourceid>FETCH-LOGICAL-c424t-11aa067168e52f86e52f74d4de82b1dc8be064eabf0d60a42a013cfb7c9d84543</originalsourceid><addsrcrecordid>eNpdUU1r3DAUFKWh2aS991QEvfTi7dOXV74E0nwWAjk0pb0JWX7OarGljaQN7L-P80mSy5vDmxlmGEK-MphzJpj6Oa7GzIHpOVdzOW8-kBlnGiopQHwkMyYaWbFG_d8lezmvAITitf5EdgU0TGrFZuTqn01l6UP1p9iUtnQCH3y4pn1MtCyRHmNBV3wMNPb0HAfvYmtdwUTX2yEmT32gZzaX5B395eM6e8yfyU5vh4xfnnCf_D09uTo6ry4uz34fHV5UTnJZKsashXrBao2K97q-vwvZyQ41b1nndItQS7RtD10NVnILTLi-Xbim01JJsU8OHn3Xm3bEzmEoyQ5mnfxo09ZE683bT_BLcx1vTS05V2IxGfx4MkjxZoO5mNFnh8NgA8ZNNpw1WtWghJ6o399RV3GTwlTPcAFcNFNUmFjwyHIp5pywfwnDwDxMZp4nM1wZaZpJ8u11iRfB80biDj2_k-Q</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2302390640</pqid></control><display><type>article</type><title>Warthin-Starry Staining for the Detection of Helicobacter pylori in Gastric Biopsies</title><source>PubMed Central</source><creator>Farouk, Wirda Indah ; Hassan, Nur Hidayah ; Ismail, Teh Rasyidah ; Daud, Intan Sufinaz ; Mohammed, Fazarina</creator><creatorcontrib>Farouk, Wirda Indah ; Hassan, Nur Hidayah ; Ismail, Teh Rasyidah ; Daud, Intan Sufinaz ; Mohammed, Fazarina ; Universiti Kuala Lumpur, Institute of Medical Science & Technology (UNIKL MESTECH), 43000 Kajang, Selangor, Malaysia ; Pathology Department, Universiti Kebangsaan Malaysia Medical Center (UKMMC), 56000 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia</creatorcontrib><description>Warthin-Starry (WS) staining is an ancillary stain used in the detection of
, spirochaete and other microorganisms in tissue sections. The present study aimed to determine the validity of WS stain in the confirmation of
diagnosis in gastric biopsies in comparison with anti
immunohistochemistry (IHC) staining.
This study involved 104 cases of gastric biopsies that were previously subjected to WS staining. All cases involved retrieval of formalin-fixed paraffin-embedded (FFPE) gastric biopsies that were re-cut, subjected to anti
IHC staining and reviewed blindly by a pathologist. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of WS as compared to IHC were calculated.
In this study, WS stain was less sensitive in detecting
. The sensitivity, specificity, PPV and NPV for WS stain were 50.0%, 92.4%, 79.2% and 76.3%, respectively.
The sensitivity of WS stain in the histopathology laboratory was lower than that described previously. Several external factors that might influence the results were identified. However, sufficient information on patients' history of treatment and medication would be required for the diagnosis or confirmation of the presence of
in gastric biopsies by WS staining.</description><identifier>ISSN: 1394-195X</identifier><identifier>EISSN: 2180-4303</identifier><identifier>DOI: 10.21315/mjms2018.25.4.9</identifier><identifier>PMID: 30914851</identifier><language>eng</language><publisher>Malaysia: Universiti Sains Malaysia Press</publisher><subject>Archives & records ; Bacteria ; Biopsy ; Endoscopy ; Gastroenterology ; Hepatology ; Histopathology ; Identification ; Infections ; Laboratories ; Microorganisms ; Original ; Phenols ; Stains & staining ; Stomach</subject><ispartof>The Malaysian journal of medical sciences, 2018-07, Vol.25 (4), p.92-99</ispartof><rights>Copyright Universiti Sains Malaysia Press Jul/Aug 2018</rights><rights>Penerbit Universiti Sains Malaysia, 2018 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-11aa067168e52f86e52f74d4de82b1dc8be064eabf0d60a42a013cfb7c9d84543</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6422537/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6422537/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30914851$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Farouk, Wirda Indah</creatorcontrib><creatorcontrib>Hassan, Nur Hidayah</creatorcontrib><creatorcontrib>Ismail, Teh Rasyidah</creatorcontrib><creatorcontrib>Daud, Intan Sufinaz</creatorcontrib><creatorcontrib>Mohammed, Fazarina</creatorcontrib><creatorcontrib>Universiti Kuala Lumpur, Institute of Medical Science & Technology (UNIKL MESTECH), 43000 Kajang, Selangor, Malaysia</creatorcontrib><creatorcontrib>Pathology Department, Universiti Kebangsaan Malaysia Medical Center (UKMMC), 56000 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia</creatorcontrib><title>Warthin-Starry Staining for the Detection of Helicobacter pylori in Gastric Biopsies</title><title>The Malaysian journal of medical sciences</title><addtitle>Malays J Med Sci</addtitle><description>Warthin-Starry (WS) staining is an ancillary stain used in the detection of
, spirochaete and other microorganisms in tissue sections. The present study aimed to determine the validity of WS stain in the confirmation of
diagnosis in gastric biopsies in comparison with anti
immunohistochemistry (IHC) staining.
This study involved 104 cases of gastric biopsies that were previously subjected to WS staining. All cases involved retrieval of formalin-fixed paraffin-embedded (FFPE) gastric biopsies that were re-cut, subjected to anti
IHC staining and reviewed blindly by a pathologist. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of WS as compared to IHC were calculated.
In this study, WS stain was less sensitive in detecting
. The sensitivity, specificity, PPV and NPV for WS stain were 50.0%, 92.4%, 79.2% and 76.3%, respectively.
The sensitivity of WS stain in the histopathology laboratory was lower than that described previously. Several external factors that might influence the results were identified. However, sufficient information on patients' history of treatment and medication would be required for the diagnosis or confirmation of the presence of
in gastric biopsies by WS staining.</description><subject>Archives & records</subject><subject>Bacteria</subject><subject>Biopsy</subject><subject>Endoscopy</subject><subject>Gastroenterology</subject><subject>Hepatology</subject><subject>Histopathology</subject><subject>Identification</subject><subject>Infections</subject><subject>Laboratories</subject><subject>Microorganisms</subject><subject>Original</subject><subject>Phenols</subject><subject>Stains & staining</subject><subject>Stomach</subject><issn>1394-195X</issn><issn>2180-4303</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdUU1r3DAUFKWh2aS991QEvfTi7dOXV74E0nwWAjk0pb0JWX7OarGljaQN7L-P80mSy5vDmxlmGEK-MphzJpj6Oa7GzIHpOVdzOW8-kBlnGiopQHwkMyYaWbFG_d8lezmvAITitf5EdgU0TGrFZuTqn01l6UP1p9iUtnQCH3y4pn1MtCyRHmNBV3wMNPb0HAfvYmtdwUTX2yEmT32gZzaX5B395eM6e8yfyU5vh4xfnnCf_D09uTo6ry4uz34fHV5UTnJZKsashXrBao2K97q-vwvZyQ41b1nndItQS7RtD10NVnILTLi-Xbim01JJsU8OHn3Xm3bEzmEoyQ5mnfxo09ZE683bT_BLcx1vTS05V2IxGfx4MkjxZoO5mNFnh8NgA8ZNNpw1WtWghJ6o399RV3GTwlTPcAFcNFNUmFjwyHIp5pywfwnDwDxMZp4nM1wZaZpJ8u11iRfB80biDj2_k-Q</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Farouk, Wirda Indah</creator><creator>Hassan, Nur Hidayah</creator><creator>Ismail, Teh Rasyidah</creator><creator>Daud, Intan Sufinaz</creator><creator>Mohammed, Fazarina</creator><general>Universiti Sains Malaysia Press</general><general>Penerbit Universiti Sains Malaysia</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BVBZV</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180701</creationdate><title>Warthin-Starry Staining for the Detection of Helicobacter pylori in Gastric Biopsies</title><author>Farouk, Wirda Indah ; 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, spirochaete and other microorganisms in tissue sections. The present study aimed to determine the validity of WS stain in the confirmation of
diagnosis in gastric biopsies in comparison with anti
immunohistochemistry (IHC) staining.
This study involved 104 cases of gastric biopsies that were previously subjected to WS staining. All cases involved retrieval of formalin-fixed paraffin-embedded (FFPE) gastric biopsies that were re-cut, subjected to anti
IHC staining and reviewed blindly by a pathologist. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of WS as compared to IHC were calculated.
In this study, WS stain was less sensitive in detecting
. The sensitivity, specificity, PPV and NPV for WS stain were 50.0%, 92.4%, 79.2% and 76.3%, respectively.
The sensitivity of WS stain in the histopathology laboratory was lower than that described previously. Several external factors that might influence the results were identified. However, sufficient information on patients' history of treatment and medication would be required for the diagnosis or confirmation of the presence of
in gastric biopsies by WS staining.</abstract><cop>Malaysia</cop><pub>Universiti Sains Malaysia Press</pub><pmid>30914851</pmid><doi>10.21315/mjms2018.25.4.9</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1394-195X |
| ispartof | The Malaysian journal of medical sciences, 2018-07, Vol.25 (4), p.92-99 |
| issn | 1394-195X 2180-4303 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6422537 |
| source | PubMed Central |
| subjects | Archives & records Bacteria Biopsy Endoscopy Gastroenterology Hepatology Histopathology Identification Infections Laboratories Microorganisms Original Phenols Stains & staining Stomach |
| title | Warthin-Starry Staining for the Detection of Helicobacter pylori in Gastric Biopsies |
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