CRISPR/Cas9 – An evolving biological tool kit for cancer biology and oncology

The development of genetic engineering in the 1970s marked a new frontier in genome-editing technology. Gene-editing technologies have provided a plethora of benefits to the life sciences. The c lustered r egularly i nterspaced s hort p alindromic r epeats/CRISPR associated protein 9 (CRISPR/ Cas9)...

Full description

Saved in:
Bibliographic Details
Published in:NPJ precision oncology 2019-03, Vol.3 (1), p.8, Article 8
Main Authors: Tian, Xueli, Gu, Tingxuan, Patel, Satyananda, Bode, Ann M., Lee, Mee-Hyun, Dong, Zigang
Format: Article
Language:English
Subjects:
Cancer
Cancer Research
CRISPR
Gene Therapy
Genetic engineering
Genome editing
Genomes
Human Genetics
Internal Medicine
Medicine
Medicine & Public Health
Oncology
Review
Review Article
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The development of genetic engineering in the 1970s marked a new frontier in genome-editing technology. Gene-editing technologies have provided a plethora of benefits to the life sciences. The c lustered r egularly i nterspaced s hort p alindromic r epeats/CRISPR associated protein 9 (CRISPR/ Cas9) system is a versatile technology that provides the ability to add or remove DNA in the genome in a sequence-specific manner. Serious efforts are underway to improve the efficiency of CRISPR/Cas9 targeting and thus reduce off-target effects. Currently, various applications of CRISPR/Cas9 are used in cancer biology and oncology to perform robust site-specific gene editing, thereby becoming more useful for biological and clinical applications. Many variants and applications of CRISPR/Cas9 are being rapidly developed. Experimental approaches that are based on CRISPR technology have created a very promising tool that is inexpensive and simple for developing effective cancer therapeutics. This review discusses diverse applications of CRISPR-based gene-editing tools in oncology and potential future cancer therapies.
ISSN:2397-768X
2397-768X
DOI:10.1038/s41698-019-0080-7