Porphyromonas, a potential predictive biomarker of Pseudomonas aeruginosa pulmonary infection in cystic fibrosis

• Published in: BMJ open respiratory research 2019, Vol.6 (1), p.e000374-e000374
• Main Authors: Keravec, Marlène, Mounier, Jérôme, Guilloux, Charles-Antoine, Fangous, Marie-Sarah, Mondot, Stanislas, Vallet, Sophie, Gouriou, Stéphanie, Le Berre, Rozenn, Rault, Gilles, Férec, Claude, Barbier, Georges, Lepage, Patricia, Héry-Arnaud, Geneviève
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Antibiotics; Bacterial infections; Biomarkers; Child; Cystic Fibrosis; Cystic Fibrosis - complications; Cystic Fibrosis - immunology; DNA, Bacterial - isolation & purification; Female; Follow-Up Studies; France; Humans; Life Sciences; Male; Medical prognosis; Medical screening; Metagenomics; Microbiota; Microbiota - genetics; Microbiota - immunology; Pathogens; Patients; Porphyromonas - genetics; Porphyromonas - immunology; Porphyromonas - isolation & purification; Precision medicine; Predictive Value of Tests; Prognosis; Prospective Studies; Pseudomonas aeruginosa - immunology; Pseudomonas aeruginosa - isolation & purification; Pseudomonas Infections - diagnosis; Pseudomonas Infections - etiology; Pseudomonas Infections - microbiology; Real-Time Polymerase Chain Reaction; Respiratory Mucosa - microbiology; RNA, Ribosomal, 16S - genetics; Sputum - microbiology; Symbiosis - immunology; Young Adult
• ISSN: 2052-4439; 2052-4439
• DOI: 10.1136/bmjresp-2018-000374

Introduction Pseudomonas aeruginosa pulmonary infections are the primary cause of morbi-mortality in patients with cystic fibrosis (CF). In this cohort study, the objective was to identify candidate biomarkers of P. aeruginosa infection within the airway microbiota.MethodsA 3-year prospective multicentre study (PYOMUCO study) was conducted in Western France and included patients initially P. aeruginosa free for at least 1 year. A 16S-targeted metagenomics approach was applied on iterative sputum samples of a first set of patients (n=33). The composition of airway microbiota was compared according to their P. aeruginosa status at the end of the follow-up (colonised vs non-colonised), and biomarkers associated with P. aeruginosa were screened. In a second step, the distribution of a candidate biomarker according to the two groups of patients was verified by qPCR on a second set of patients (n=52) coming from the same cohort and its load quantified throughout the follow-up.Results Porphyromonas (mainly P. catoniae) was found to be an enriched phylotype in patients uninfected by P. aeruginosa (p