Osteocyte viability and bone density in cadmium chloride‐induced osteoporosis ameliorated with Pilostigma thonningii stem bark‐extracted D‐3‐O‐methy‐chiroinositol

• Published in: Animal models and experimental medicine 2019-03, Vol.2 (1), p.25-33
• Main Authors: Uwagie‐Ero, Edwin Aihanuwa, Abiaezute, Clifford Nwabugwu, Nwaehujor, Chinaka O., Nnaji, Theophilus Okafor, Odigie, Eugene Amienwanlen, Ekeolu, Oyetunde Kazeem, Ocheja, Ohiemi Benjamin
• Format: Article
• Language: English
• Subjects: Bark; Bone density; Bones; Cadmium; Cadmium chloride; Calcium phosphates; Chloride; Drinking water; D‐3‐O‐methyl‐chiroinositol; Femur; Heavy metals; Laboratory animals; Magnesium; Medical research; Mineralization; Original; Osteocytes; Osteoporosis; Oxidative stress; Phosphorus; Poisoning; Proline; Rodents; Studies; Tibia; toxicity, amelioration; Zinc
• ISSN: 2576-2095; 2096-5451; 2576-2095
• DOI: 10.1002/ame2.12054

Background This study examined the ameliorative effect of D‐3‐O‐methyl‐chiroinositol, isolated from the stem bark of Piliostigma thonningii, on cadmium chloride‐induced osteoporosis in male Wistar rats. Methods Thirty‐six rats were assigned to three treatment groups (n = 12). Group A (2 mL distilled water), group B: (2.5 mg/kg b.w. CdCl2) and group C: (2.5 mg/kg b.w. CdCl2 and D‐3‐O‐methyl‐chiroinositol 2 mg/kg b.w.). Bone ash, calcium, phosphate, magnesium, and zinc content, as well as bone histological changes were determined at the end of months 1, 2, and 3. Results There were significant differences (P ≤ 0.05) in the weight of the cervical, tibia, and femoral bones in all groups. The serum concentration of CdCl2 was significantly different across the three groups with time. There was significant variation (P