Pseudomonas aeruginosa keratitis: passive immunotherapy with antibodies raised against divalent flagellin

infections such as keratitis are considered among the major health problems worldwide due to the complexity of pathogenesis and antibiotic resistance crisis, thus, finding new effective approaches for prevention and treatment of the infections seem to be still vital. In this report, we aimed to inve...

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Bibliographic Details
Published in:Iranian journal of basic medical sciences 2019-01, Vol.22 (1), p.58-64
Main Authors: Mahin Samadi, Pariya, Gerami, Parmida, Elmi, Ali, Khanaki, Korosh, Faezi, Sobhan
Format: Article
Language:English
Subjects:
Antibiotics
Bacteria
Cornea
Immunoglobulins
Immunotherapy
Laboratories
Molecular weight
Original
Pathogenesis
Pathogens
Research centers
Urinary tract infections
Urogenital system
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Summary:infections such as keratitis are considered among the major health problems worldwide due to the complexity of pathogenesis and antibiotic resistance crisis, thus, finding new effective approaches for prevention and treatment of the infections seem to be still vital. In this report, we aimed to investigate the therapeutic effects of topical administration of the antibodies against type a and b-flagellin (FLA and FLB) in keratitis model of infection in mice. Scratched corneas of mice were treated with approximately 10 CFUs/eye of PAK and/or PAO1 strains of . Specific IgG to FLA, FLB or divalent flagellin were topically applied to the infected corneas for 20 min, 24, and 36 hr post-infection. The bacterial burden and myeloperoxidase activity (as a marker for polymorphonuclears (PMNs) infiltration) were determined in the corneas. The biological activity of the anti-FLA and FLB IgG was evaluated by opsonophagocytosis test. Compared to other treated corneas, divalent anti-flagellin IgG treatment showed a significant decrease in the bacterial CFUs and myeloperoxidase activity in the infected corneas (
ISSN:2008-3866
2008-3874
DOI:10.22038/ijbms.2018.31499.7643