Proteome Analysis of Human Neutrophil Granulocytes From Patients With Monogenic Disease Using Data-independent Acquisition[S]

• Published in: Molecular & cellular proteomics 2019-04, Vol.18 (4), p.760-772
• Main Authors: Grabowski, Piotr, Hesse, Sebastian, Hollizeck, Sebastian, Rohlfs, Meino, Behrends, Uta, Sherkat, Roya, Tamary, Hannah, Ünal, Ekrem, Somech, Raz, Patıroğlu, Türkan, Canzar, Stefan, van der Werff Ten Bosch, Jutte, Klein, Christoph, Rappsilber, Juri
• Format: Article
• Language: English
• Subjects: Base Sequence; data-independent acquisition; Diagnostic; Disease - genetics; Humans; Immunology; neutrophil granulocyte; Neutrophils - metabolism; Omics; Personalized medicine; primary immunodeficiency diseases; Proteogenomics; Proteome - metabolism; Proteomics; RNA, Messenger - genetics; RNA, Messenger - metabolism; systems medicine; whole exome sequencing
• ISSN: 1535-9476; 1535-9484
• DOI: 10.1074/mcp.RA118.001141

Data-independent acquisition proteomics was used to study proteome changes of naive human neutrophils in rare monogenic diseases affecting their functions. Neutrophils of patients with mutations in the neutrophil elastase gene ELANE demonstrated global proteome dysregulation, whereas chronic granulomatous disease and leukocyte adhesion deficiency had modest effects on the respective neutrophil proteomes. Proteomics then guided targeted genetic assays to resolve two clinical cases with undetermined genetic causes, highlighting the usefulness of mass spectrometry-based clinical diagnostics. [Display omitted] Highlights •Data-independent acquisition proteomics analysis of naive neutrophils from patients with rare monogenic diseases.•Proteomics analysis helps guide targeted genetic diagnostics of patients for which routine clinical diagnostics proved inconclusive. Neutrophil granulocytes are critical mediators of innate immunity and tissue regeneration. Rare diseases of neutrophil granulocytes may affect their differentiation and/or functions. However, there are very few validated diagnostic tests assessing the functions of neutrophil granulocytes in these diseases. Here, we set out to probe omics analysis as a novel diagnostic platform for patients with defective differentiation and function of neutrophil granulocytes. We analyzed highly purified neutrophil granulocytes from 68 healthy individuals and 16 patients with rare monogenic diseases. Cells were isolated from fresh venous blood (purity >99%) and used to create a spectral library covering almost 8000 proteins using strong cation exchange fractionation. Patient neutrophil samples were then analyzed by data-independent acquisition proteomics, quantifying 4154 proteins in each sample. Neutrophils with mutations in the neutrophil elastase gene ELANE showed large proteome changes that suggest these mutations may affect maturation of neutrophil granulocytes and initiate misfolded protein response and cellular stress mechanisms. In contrast, only few proteins changed in patients with leukocyte adhesion deficiency (LAD) and chronic granulomatous disease (CGD). Strikingly, neutrophil transcriptome analysis showed no correlation with its proteome. In case of two patients with undetermined genetic causes, proteome analysis guided the targeted genetic diagnostics and uncovered the underlying genomic mutations. Data-independent acquisition proteomics may help to define novel pathomechanisms in neutrophil diseases a