Development of electrochemical biosensor based on CNT–Fe3O4 nanocomposite to determine formaldehyde adulteration in orange juice

• Published in: Journal of food science and technology 2019-04, Vol.56 (4), p.1829-1840
• Main Authors: Kundu, Monika, Bhardwaj, Hema, Pandey, Manoj Kumar, Krishnan, Prameela, Kotnala, R. K., Sumana, Gajjala
• Format: Article
• Language: English
• Subjects: Agribusiness; Agricultural production; Biosensors; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Citrus fruits; Electrochemistry; Food; Food chains; Food safety; Food Science; Foodborne diseases; Formaldehyde; Formaldehyde dehydrogenase; Fruit juices; Iron oxides; Juices; Multi wall carbon nanotubes; Nanocomposites; Nanotechnology; Nanotubes; Nutrition; Oranges; Original; Original Article; Outbreaks
• ISSN: 0022-1155; 0975-8402
• DOI: 10.1007/s13197-019-03635-7

An electrochemical biosensor was developed to determine formaldehyde (HCHO) adulteration commonly found in food. The current responses of various electrodes based on multiwalled carbon nanotubes (CNTs) and synthesized nanocomposite (CNT–Fe 3 O 4 ) were measured using cyclic voltammetry. The nanocomposite based biosensor shows comparatively high sensitivity (527 µA mg/L −1  cm −2 ), low detection limit (0.05 mg/L) in linear detection range 0.05–0.5 mg/L for formaldehyde detection using formaldehyde dehydrogenase (FDH) enzyme. In real sample analysis, the low obtained RSD values (less than 1.79) and good recovery rates (more than 90%) signify an efficient and precise sensor for the selective quantification of formaldehyde in orange juice. The developed biosensor has future implications for determining formaldehyde adulteration in citrus fruit juices and other liquid foods in agri-food chain to further resolve global food safety concerns, control unethical business practices of adulteration and reduce the widespread food borne illness outbreaks.