Effects of local insulin delivery on subperiosteal angiogenesis and mineralized tissue formation during fracture healing

Local insulin delivery has been shown to improve osseous healing in diabetic animals. The purpose of this study was to quantify the effects of local intramedullary delivery of saline or Ultralente insulin (UL) on various fracture healing parameters using an in vivo non‐diabetic BB Wistar rat model....

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Bibliographic Details
Published in:Journal of orthopaedic research 2013-05, Vol.31 (5), p.783-791
Main Authors: Paglia, David N., Wey, Aaron, Breitbart, Eric A., Faiwiszewski, Jonathan, Mehta, Siddhant K., Al-Zube, Loay, Vaidya, Swaroopa, Cottrell, Jessica A., Graves, Dana, Benevenia, Joseph, O'Connor, J. Patrick, Lin, Sheldon S.
Format: Article
Language:English
Subjects:
Animals
BB wistar rat
Biomechanical Phenomena - drug effects
Biomechanical Phenomena - physiology
Blood Vessels - metabolism
bone regeneration
Calcification, Physiologic - drug effects
Calcification, Physiologic - physiology
Cell Proliferation - drug effects
Diaphyses - diagnostic imaging
Diaphyses - drug effects
Diaphyses - physiology
Disease Models, Animal
Female
Femoral Fractures - diagnostic imaging
Femoral Fractures - drug therapy
Femoral Fractures - physiopathology
Femur - diagnostic imaging
Femur - drug effects
Femur - physiology
fracture
Fracture Healing - drug effects
Fracture Healing - physiology
Hypoglycemic Agents - pharmacology
Injections, Intralesional
insulin
Insulin, Ultralente - pharmacology
local delivery
Male
Neovascularization, Physiologic - drug effects
Neovascularization, Physiologic - physiology
Platelet Endothelial Cell Adhesion Molecule-1 - metabolism
Radiography
Random Allocation
Rats
Rats, Inbred BB
Vascular Endothelial Growth Factor A - metabolism
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Summary:Local insulin delivery has been shown to improve osseous healing in diabetic animals. The purpose of this study was to quantify the effects of local intramedullary delivery of saline or Ultralente insulin (UL) on various fracture healing parameters using an in vivo non‐diabetic BB Wistar rat model. Quantitation of local insulin levels showed a rapid release of insulin from the fractured femora, demonstrating complete release at 2 days. RT‐PCR analysis revealed that the expression of early osteogenic markers (Col1α2, osteopontin) was significantly enhanced with UL treatment when compared with saline controls (p 
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.22288