CD140b (PDGFRβ) Signaling in Adipose-Derived Stem Cells Mediates Angiogenic Behavior of Retinal Endothelial Cells

Adipose-derived stem cells (ASCs) are multipotent mesenchymal progenitor cells that have functional and phenotypic overlap with pericytes lining microvessels in adipose tissue. The role of CD140b [platelet-derived growth factor receptor-β (PDGFR-β)], a constitutive marker expressed by ASCs, in the a...

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Bibliographic Details
Published in:Regenerative engineering and translational medicine 2019-03, Vol.5 (1), p.1-9
Main Authors: Periasamy, Ramesh, Elshaer, Sally L., Gangaraju, Rajashekhar
Format: Article
Language:English
Subjects:
Adhesion tests
Adipose tissue
Biomaterials
Biomedical Engineering and Bioengineering
Cells (biology)
Chemistry and Materials Science
Coating effects
Cytokines
Endothelial cells
Growth factors
Human behavior
In vivo methods and tests
Invited Papers— Tao L. Lowe
Laminin
Materials Science
Migration
Network formation
Platelet-derived growth factor
Proteins
Reagents
Regeneration
Regenerative Medicine/Tissue Engineering
Reinjection
Signaling
Stem cells
Studies
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Summary:Adipose-derived stem cells (ASCs) are multipotent mesenchymal progenitor cells that have functional and phenotypic overlap with pericytes lining microvessels in adipose tissue. The role of CD140b [platelet-derived growth factor receptor-β (PDGFR-β)], a constitutive marker expressed by ASCs, in the angiogenic behavior of human retinal endothelial cells (HREs) is not known. CD140b was knocked down in ASCs using targeted siRNA and Lipofectamine transfection protocol. Both CD140b+ and CD140b− ASCs were tested for their proliferation (WST-1 reagent), adhesion (laminin-1-coated plates), and migration (wound-scratch assay). Angiogenic effect of CD140b+ and CD140b− ASCs on HREs was examined by co-culturing ASCs:HREs in 12:1 ratio for 6 days followed by visualization of vascular network by isolectin B4 staining. The RayBio ® Membrane-Based Antibody Array was used to assess differences in human cytokines released by CD140b+ or CD140b− ASCs. Knockdown of CD140b in ASCs resulted in a significant 50% decrease in proliferation rate, 25% decrease in adhesion ability to laminin-1, and 50% decrease in migration rate, as compared to CD140b+ ASCs. Direct contact of ASCs expressing CD140b+ with HREs resulted in robust vascular network formation that was significantly reduced with using CD140b− ASCs. Of the 80 proteins tested, 45 proteins remained unchanged (> 0.5-–
ISSN:2364-4133
2364-4141
DOI:10.1007/s40883-018-0068-9