MicroRNA-26a reduces synovial inflammation and cartilage injury in osteoarthritis of knee joints through impairing the NF-κB signaling pathway

• Published in: Bioscience reports 2019-04, Vol.39 (4)
• Main Authors: Zhao, Zhi, Dai, Xiu-Song, Wang, Zhi-Yan, Bao, Zheng-Qi, Guan, Jian-Zhong
• Format: Article
• Language: English
• Subjects: Animals; Female; Inflammation - immunology; Inflammation - pathology; Male; MicroRNAs - analysis; MicroRNAs - immunology; NF-kappa B - immunology; Osteoarthritis, Knee - immunology; Osteoarthritis, Knee - pathology; Rats; Rats, Sprague-Dawley; Signal Transduction; Synovial Membrane - immunology; Synovial Membrane - pathology; Synoviocytes - immunology; Synoviocytes - pathology
• ISSN: 0144-8463; 1573-4935
• DOI: 10.1042/BSR20182025

Inflammation is closely implicated in the process of osteoarthritis (OA) and affects disease progression and pain. Herein, the present study explored the effect of microRNA-26a (miR-26a) on the synovial inflammation and cartilage injury in OA, with the involvement with the NF-κB signaling pathway. Rat models of OA were established by anterior cruciate ligament transection, which were then treated with miR-26a mimics/inhibitors or BMS-345541 (inhibitor of NF-κB pathway). The expression of miR-26a and activator proteins of NF-κB pathway (P-IκBα and P-P65) in synovial tissues was determined. Hematoxylin-eosin (HE) staining was adopted to observe pathological changes of knee joints, synovial tissues, and cartilage of femoral condyle. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining was used to detect the apoptosis of synoviocytes and chondrocytes. Poorly expressed miR-26a and increased protein levels of P-IκBα and P-P65 were identified in synovial tissues of OA rats. Besides, OA rats showed obvious synovial tissue hyperplasia, inflammation and cartilage injury of femoral condyle, as well as increased inflammation and cartilage injury scores, and apoptosis of synoviocytes and chondrocytes. In response to miR-26a mimics, protein levels of P-IκBα and P-P65 were reduced; meanwhile, synovial tissue hyperplasia, inflammation and cartilage injury of femoral condyle were ameliorated, with decreased inflammation and cartilage injury scores, and apoptosis of synoviocytes and chondrocytes. MiR-26a suppressed the activation of the NF-κB signaling pathway, by which mechanism the synovial inflammation and cartilage injury in OA rats were alleviated.