Overarching Immunodominance Patterns and Substantial Diversity in Specificity and Functionality in the Circulating Human Influenza A and B Virus-Specific CD4+ T-Cell Repertoire

There is limited information on the antigen specificity and functional potential of the influenza virus-specific CD4+ T-cell repertoire in humans. Here, enzyme-linked immunospot assays were used to examine circulating CD4+ T-cell specificities for influenza virus directly ex vivo in healthy adults....

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 2018-08, Vol.218 (7), p.1169-1174
Main Authors: Richards, Katherine A, Treanor, John J, Nayak, Jennifer L, Sant, Andrea J
Format: Article
Language:English
Subjects:
CD4-Positive T-Lymphocytes - immunology
Genetic Variation
Humans
Immunodominant Epitopes - immunology
Influenza A virus - genetics
Influenza A virus - immunology
Influenza B virus - genetics
Influenza B virus - immunology
Influenza, Human - immunology
Major and Brief Reports
Sensitivity and Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:There is limited information on the antigen specificity and functional potential of the influenza virus-specific CD4+ T-cell repertoire in humans. Here, enzyme-linked immunospot assays were used to examine circulating CD4+ T-cell specificities for influenza virus directly ex vivo in healthy adults. Our studies revealed CD4+ T-cell reactivity to multiple influenza virus proteins, including hemagglutinins, neuraminidases, M1 proteins, and nucleoproteins. Unexpectedly, the immunodominance hierarchies and functional potential of cells reactive toward influenza A virus were distinct from those toward influenza B virus. We also identified influenza virus-specific cells producing granzyme B. Our findings revealed individual and virus-specific patterns that may differentially poise humans to respond to infection or vaccination.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/jiy288