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miRNAs expression of oral squamous cell carcinoma patients: Validation of two putative biomarkers
microRNA expression patterns have provided new directions in the search of biomarkers with prognostic value and even in the search of novel therapeutic targets for several neoplasms. Specifically, miRNAs profiling in oral squamous cell carcinoma (OSCC) represents a web of intrigue in the study of or...
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Published in: | Medicine (Baltimore) 2019-03, Vol.98 (13), p.e14922-e14922 |
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creator | Chamorro Petronacci, Cintia Micaela Pérez-Sayáns, Mario Padín Iruegas, María Elena Suárez Peñaranda, José M. Lorenzo Pouso, Alejandro Ismael Blanco Carrión, Andrés García García, Abel |
description | microRNA expression patterns have provided new directions in the search of biomarkers with prognostic value and even in the search of novel therapeutic targets for several neoplasms. Specifically, miRNAs profiling in oral squamous cell carcinoma (OSCC) represents a web of intrigue in the study of oral carcinogenesis. The objective of the present study was twofold:The first study phase comprised case-control groups: A) 8 OSCC-affected patients and 8 healthy controls. Microarray technology (Affymetrix miRNA Array Plate 4.1) was used for miRNAs expression profile. Deregulated miRNAs were studied using Diana Tools miRPath 3.0 to associate miRNA targets with molecular pathways via Kyoto Encyclopedia of Genes and Genomes (KEGG). In a second phase, 2 miRNAs chosen for the subsequent RT-qPCR validation were studied in a second OSSC cohort (n = 8).Microarray analysis identified 80 deregulated miRNAs (35 over-expressed and 45 under-expressed). Two miRNAs (miR-497-5p and miR-4417) were chosen for further validation via RT-qPCR. Prognostic analysis did not ascertain relevant relation between miR-497-5p or miR-4417 expression and clinical or pathological parameters, except high miR-4417 in the case of nodular affectation (P = .035) and diminished miR-497-5p radiotherapy-treated patients (P = .05). KEGG analysis revealed that deregulated miRNAs were implicated in several biological pathways such as Proteoglycans in cancer.Our data suggest an altered miRNAs profiling in OSCC-affected patients. We have verified the altered expression of miR-497-5p and miR-4417 in OSCC samples and related the deregulated miRNAs with the 'proteoglycans in cancer' pathway. Further longitudinal studies with large samples are warranted to confirm the present findings. |
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Specifically, miRNAs profiling in oral squamous cell carcinoma (OSCC) represents a web of intrigue in the study of oral carcinogenesis. The objective of the present study was twofold:The first study phase comprised case-control groups: A) 8 OSCC-affected patients and 8 healthy controls. Microarray technology (Affymetrix miRNA Array Plate 4.1) was used for miRNAs expression profile. Deregulated miRNAs were studied using Diana Tools miRPath 3.0 to associate miRNA targets with molecular pathways via Kyoto Encyclopedia of Genes and Genomes (KEGG). In a second phase, 2 miRNAs chosen for the subsequent RT-qPCR validation were studied in a second OSSC cohort (n = 8).Microarray analysis identified 80 deregulated miRNAs (35 over-expressed and 45 under-expressed). Two miRNAs (miR-497-5p and miR-4417) were chosen for further validation via RT-qPCR. Prognostic analysis did not ascertain relevant relation between miR-497-5p or miR-4417 expression and clinical or pathological parameters, except high miR-4417 in the case of nodular affectation (P = .035) and diminished miR-497-5p radiotherapy-treated patients (P = .05). KEGG analysis revealed that deregulated miRNAs were implicated in several biological pathways such as Proteoglycans in cancer.Our data suggest an altered miRNAs profiling in OSCC-affected patients. We have verified the altered expression of miR-497-5p and miR-4417 in OSCC samples and related the deregulated miRNAs with the 'proteoglycans in cancer' pathway. Further longitudinal studies with large samples are warranted to confirm the present findings.</description><identifier>ISSN: 0025-7974</identifier><identifier>EISSN: 1536-5964</identifier><identifier>DOI: 10.1097/MD.0000000000014922</identifier><identifier>PMID: 30921188</identifier><language>eng</language><publisher>United States: the Author(s). Published by Wolters Kluwer Health, Inc</publisher><subject>Biomarkers, Tumor - genetics ; Carcinogenesis - genetics ; Carcinogenesis - pathology ; Carcinoma, Squamous Cell - epidemiology ; Carcinoma, Squamous Cell - genetics ; Carcinoma, Squamous Cell - mortality ; Carcinoma, Squamous Cell - pathology ; Female ; Gene Expression Profiling - methods ; Humans ; Incidence ; Male ; Microarray Analysis - methods ; MicroRNAs - genetics ; Mouth Neoplasms - epidemiology ; Mouth Neoplasms - genetics ; Mouth Neoplasms - mortality ; Mouth Neoplasms - pathology ; Neoplasm Staging ; Observational Study ; Prognosis ; Proteoglycans - metabolism ; Survival Rate</subject><ispartof>Medicine (Baltimore), 2019-03, Vol.98 (13), p.e14922-e14922</ispartof><rights>the Author(s). Published by Wolters Kluwer Health, Inc.</rights><rights>Copyright © 2019 the Author(s). Published by Wolters Kluwer Health, Inc. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2854-f9edb04cd8b6e2f5baf737237d46726a5d8892e7aaaf3c18460c21ebea4163f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456104/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456104/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30921188$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chamorro Petronacci, Cintia Micaela</creatorcontrib><creatorcontrib>Pérez-Sayáns, Mario</creatorcontrib><creatorcontrib>Padín Iruegas, María Elena</creatorcontrib><creatorcontrib>Suárez Peñaranda, José M.</creatorcontrib><creatorcontrib>Lorenzo Pouso, Alejandro Ismael</creatorcontrib><creatorcontrib>Blanco Carrión, Andrés</creatorcontrib><creatorcontrib>García García, Abel</creatorcontrib><title>miRNAs expression of oral squamous cell carcinoma patients: Validation of two putative biomarkers</title><title>Medicine (Baltimore)</title><addtitle>Medicine (Baltimore)</addtitle><description>microRNA expression patterns have provided new directions in the search of biomarkers with prognostic value and even in the search of novel therapeutic targets for several neoplasms. Specifically, miRNAs profiling in oral squamous cell carcinoma (OSCC) represents a web of intrigue in the study of oral carcinogenesis. The objective of the present study was twofold:The first study phase comprised case-control groups: A) 8 OSCC-affected patients and 8 healthy controls. Microarray technology (Affymetrix miRNA Array Plate 4.1) was used for miRNAs expression profile. Deregulated miRNAs were studied using Diana Tools miRPath 3.0 to associate miRNA targets with molecular pathways via Kyoto Encyclopedia of Genes and Genomes (KEGG). In a second phase, 2 miRNAs chosen for the subsequent RT-qPCR validation were studied in a second OSSC cohort (n = 8).Microarray analysis identified 80 deregulated miRNAs (35 over-expressed and 45 under-expressed). Two miRNAs (miR-497-5p and miR-4417) were chosen for further validation via RT-qPCR. Prognostic analysis did not ascertain relevant relation between miR-497-5p or miR-4417 expression and clinical or pathological parameters, except high miR-4417 in the case of nodular affectation (P = .035) and diminished miR-497-5p radiotherapy-treated patients (P = .05). KEGG analysis revealed that deregulated miRNAs were implicated in several biological pathways such as Proteoglycans in cancer.Our data suggest an altered miRNAs profiling in OSCC-affected patients. We have verified the altered expression of miR-497-5p and miR-4417 in OSCC samples and related the deregulated miRNAs with the 'proteoglycans in cancer' pathway. Further longitudinal studies with large samples are warranted to confirm the present findings.</description><subject>Biomarkers, Tumor - genetics</subject><subject>Carcinogenesis - genetics</subject><subject>Carcinogenesis - pathology</subject><subject>Carcinoma, Squamous Cell - epidemiology</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Carcinoma, Squamous Cell - mortality</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Female</subject><subject>Gene Expression Profiling - methods</subject><subject>Humans</subject><subject>Incidence</subject><subject>Male</subject><subject>Microarray Analysis - methods</subject><subject>MicroRNAs - genetics</subject><subject>Mouth Neoplasms - epidemiology</subject><subject>Mouth Neoplasms - genetics</subject><subject>Mouth Neoplasms - mortality</subject><subject>Mouth Neoplasms - pathology</subject><subject>Neoplasm Staging</subject><subject>Observational Study</subject><subject>Prognosis</subject><subject>Proteoglycans - metabolism</subject><subject>Survival Rate</subject><issn>0025-7974</issn><issn>1536-5964</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpdkNlOxCAYhYnR6Lg8gYnhBTqyFYoXJpMZt8QlMeotoZQ6aGeo0Dr69qLjzg35w3fOfzgA7GI0xEiK_YvJEP0czCQhK2CAc8qzXHK2CgYIkTwTUrANsBnjQ4KoIGwdbFAkCcZFMQB65q4vRxHalzbYGJ2fQ19DH3QD41OvZ76P0NimgUYH4-Z-pmGrO2fnXTyAd7pxVZqWom7hYdt3aX62sHQJDY82xG2wVusm2p3PewvcHh_djE-z86uTs_HoPDOkyFlWS1uViJmqKLkldV7qWqS0VFSMC8J1XhWFJFZorWtqcME4MgTb0mqGOU3sFjhc-rZ9ObOVSRHTL1QbXAryqrx26u_L3E3VvX9WnOUcI5YM6NLABB9jsPW3FiP13ri6mKj_jSfV3u-135qvihPAlsDCN13q47HpFzaoqdVNN_3wy4UkGUFYIkokyj6s6Rse9Y64</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>Chamorro Petronacci, Cintia Micaela</creator><creator>Pérez-Sayáns, Mario</creator><creator>Padín Iruegas, María Elena</creator><creator>Suárez Peñaranda, José M.</creator><creator>Lorenzo Pouso, Alejandro Ismael</creator><creator>Blanco Carrión, Andrés</creator><creator>García García, Abel</creator><general>the Author(s). Published by Wolters Kluwer Health, Inc</general><general>Wolters Kluwer Health</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20190301</creationdate><title>miRNAs expression of oral squamous cell carcinoma patients: Validation of two putative biomarkers</title><author>Chamorro Petronacci, Cintia Micaela ; Pérez-Sayáns, Mario ; Padín Iruegas, María Elena ; Suárez Peñaranda, José M. ; Lorenzo Pouso, Alejandro Ismael ; Blanco Carrión, Andrés ; García García, Abel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2854-f9edb04cd8b6e2f5baf737237d46726a5d8892e7aaaf3c18460c21ebea4163f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Biomarkers, Tumor - genetics</topic><topic>Carcinogenesis - genetics</topic><topic>Carcinogenesis - pathology</topic><topic>Carcinoma, Squamous Cell - epidemiology</topic><topic>Carcinoma, Squamous Cell - genetics</topic><topic>Carcinoma, Squamous Cell - mortality</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Female</topic><topic>Gene Expression Profiling - methods</topic><topic>Humans</topic><topic>Incidence</topic><topic>Male</topic><topic>Microarray Analysis - methods</topic><topic>MicroRNAs - genetics</topic><topic>Mouth Neoplasms - epidemiology</topic><topic>Mouth Neoplasms - genetics</topic><topic>Mouth Neoplasms - mortality</topic><topic>Mouth Neoplasms - pathology</topic><topic>Neoplasm Staging</topic><topic>Observational Study</topic><topic>Prognosis</topic><topic>Proteoglycans - metabolism</topic><topic>Survival Rate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chamorro Petronacci, Cintia Micaela</creatorcontrib><creatorcontrib>Pérez-Sayáns, Mario</creatorcontrib><creatorcontrib>Padín Iruegas, María Elena</creatorcontrib><creatorcontrib>Suárez Peñaranda, José M.</creatorcontrib><creatorcontrib>Lorenzo Pouso, Alejandro Ismael</creatorcontrib><creatorcontrib>Blanco Carrión, Andrés</creatorcontrib><creatorcontrib>García García, Abel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Medicine (Baltimore)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chamorro Petronacci, Cintia Micaela</au><au>Pérez-Sayáns, Mario</au><au>Padín Iruegas, María Elena</au><au>Suárez Peñaranda, José M.</au><au>Lorenzo Pouso, Alejandro Ismael</au><au>Blanco Carrión, Andrés</au><au>García García, Abel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miRNAs expression of oral squamous cell carcinoma patients: Validation of two putative biomarkers</atitle><jtitle>Medicine (Baltimore)</jtitle><addtitle>Medicine (Baltimore)</addtitle><date>2019-03-01</date><risdate>2019</risdate><volume>98</volume><issue>13</issue><spage>e14922</spage><epage>e14922</epage><pages>e14922-e14922</pages><issn>0025-7974</issn><eissn>1536-5964</eissn><abstract>microRNA expression patterns have provided new directions in the search of biomarkers with prognostic value and even in the search of novel therapeutic targets for several neoplasms. Specifically, miRNAs profiling in oral squamous cell carcinoma (OSCC) represents a web of intrigue in the study of oral carcinogenesis. The objective of the present study was twofold:The first study phase comprised case-control groups: A) 8 OSCC-affected patients and 8 healthy controls. Microarray technology (Affymetrix miRNA Array Plate 4.1) was used for miRNAs expression profile. Deregulated miRNAs were studied using Diana Tools miRPath 3.0 to associate miRNA targets with molecular pathways via Kyoto Encyclopedia of Genes and Genomes (KEGG). In a second phase, 2 miRNAs chosen for the subsequent RT-qPCR validation were studied in a second OSSC cohort (n = 8).Microarray analysis identified 80 deregulated miRNAs (35 over-expressed and 45 under-expressed). Two miRNAs (miR-497-5p and miR-4417) were chosen for further validation via RT-qPCR. Prognostic analysis did not ascertain relevant relation between miR-497-5p or miR-4417 expression and clinical or pathological parameters, except high miR-4417 in the case of nodular affectation (P = .035) and diminished miR-497-5p radiotherapy-treated patients (P = .05). KEGG analysis revealed that deregulated miRNAs were implicated in several biological pathways such as Proteoglycans in cancer.Our data suggest an altered miRNAs profiling in OSCC-affected patients. We have verified the altered expression of miR-497-5p and miR-4417 in OSCC samples and related the deregulated miRNAs with the 'proteoglycans in cancer' pathway. Further longitudinal studies with large samples are warranted to confirm the present findings.</abstract><cop>United States</cop><pub>the Author(s). Published by Wolters Kluwer Health, Inc</pub><pmid>30921188</pmid><doi>10.1097/MD.0000000000014922</doi><oa>free_for_read</oa></addata></record> |
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subjects | Biomarkers, Tumor - genetics Carcinogenesis - genetics Carcinogenesis - pathology Carcinoma, Squamous Cell - epidemiology Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - mortality Carcinoma, Squamous Cell - pathology Female Gene Expression Profiling - methods Humans Incidence Male Microarray Analysis - methods MicroRNAs - genetics Mouth Neoplasms - epidemiology Mouth Neoplasms - genetics Mouth Neoplasms - mortality Mouth Neoplasms - pathology Neoplasm Staging Observational Study Prognosis Proteoglycans - metabolism Survival Rate |
title | miRNAs expression of oral squamous cell carcinoma patients: Validation of two putative biomarkers |
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