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The use of laser‐based diagnostics for the rapid identification of infectious agents in human blood
Aims To investigate the use of a laser‐based method of detection as a potential diagnostic test for the rapid identification of infectious agents in human blood. Methods and Results In this study, the successful differentiation of blood spiked with viruses, bacteria or protozoan parasites to clinica...
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| Published in: | Journal of applied microbiology 2019-05, Vol.126 (5), p.1606-1617 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c4432-b7fc488dea62124f69807c7703b04eaff1173925d4b8f7297ef08b6cc3a25daf3 |
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| cites | cdi_FETCH-LOGICAL-c4432-b7fc488dea62124f69807c7703b04eaff1173925d4b8f7297ef08b6cc3a25daf3 |
| container_end_page | 1617 |
| container_issue | 5 |
| container_start_page | 1606 |
| container_title | Journal of applied microbiology |
| container_volume | 126 |
| creator | Multari, R.A. Cremers, D.A. Nelson, A. Karimi, Z. Young, S. Fisher, C. Duncan, R. |
| description | Aims
To investigate the use of a laser‐based method of detection as a potential diagnostic test for the rapid identification of infectious agents in human blood.
Methods and Results
In this study, the successful differentiation of blood spiked with viruses, bacteria or protozoan parasites to clinically relevant levels is demonstrated using six blood types (O+, O−, AB+, A+, A−, B+) using blood from different individuals with blood samples prepared in two different laboratories. Experiments were performed using various compositions of filters, experimental set‐ups and experimental parameters for spectral capture.
Conclusions
The potential for developing a laser‐based diagnostic instrument to detect the presence of parasites, bacteria and viruses in human blood capable of providing analysis results within minutes was demonstrated.
Significance and Impact of the Study
There is an ongoing need for clinical diagnostics to adapt to newly emerging agents and to screen simultaneously for multiple infectious agents. A laser‐based approach can achieve sensitive, multiplex detection with minimal sample preparation and provide rapid results (within minutes). These properties along with the flexibility to add new agent detection by simply adjusting the detection programming make it a promising tool for clinical diagnosis. |
| doi_str_mv | 10.1111/jam.14222 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6458062</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2206010142</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4432-b7fc488dea62124f69807c7703b04eaff1173925d4b8f7297ef08b6cc3a25daf3</originalsourceid><addsrcrecordid>eNp1kc1O3DAUha2KqsCURV-gssQGFgH_xU42lRCCAgJ1A2vLcewZj5J4aiet2PEIPCNP0ssMoLYS3lz73s9H5-og9IWSIwrneGn6IyoYYx_QDuWyLJhUbGt9F0VJFNtGuzkvCaGclPIT2uZEScVFuYPc7cLhKTscPe5Mdunp4bGB2uI2mPkQ8xhsxj4mPAKYzCq0OLRuGIMP1owhDs8_w-CdhceUsZnDMEMHL6beDLjpYmw_o4_edNntvdQZujs_uz29KK5_fL88PbkurBCcFY3yVlRV64xklAkv64ooqxThDRHOeE-p4jUrW9FUXrFaOU-qRlrLDTSN5zP0baO7mpretRasJNPpVQq9Sfc6mqD_nQxhoefxl5airIhkIHDwIpDiz8nlUfchW9d1ZnCwnWa0FmXNiawA3f8PXcYpDbCeZoxIQglEAtThhrIp5pycfzNDiX4OT0N4eh0esF__dv9GvqYFwPEG-B06d_--kr46udlI_gENBKV2</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2206010142</pqid></control><display><type>article</type><title>The use of laser‐based diagnostics for the rapid identification of infectious agents in human blood</title><source>Alma/SFX Local Collection</source><creator>Multari, R.A. ; Cremers, D.A. ; Nelson, A. ; Karimi, Z. ; Young, S. ; Fisher, C. ; Duncan, R.</creator><creatorcontrib>Multari, R.A. ; Cremers, D.A. ; Nelson, A. ; Karimi, Z. ; Young, S. ; Fisher, C. ; Duncan, R.</creatorcontrib><description>Aims
To investigate the use of a laser‐based method of detection as a potential diagnostic test for the rapid identification of infectious agents in human blood.
Methods and Results
In this study, the successful differentiation of blood spiked with viruses, bacteria or protozoan parasites to clinically relevant levels is demonstrated using six blood types (O+, O−, AB+, A+, A−, B+) using blood from different individuals with blood samples prepared in two different laboratories. Experiments were performed using various compositions of filters, experimental set‐ups and experimental parameters for spectral capture.
Conclusions
The potential for developing a laser‐based diagnostic instrument to detect the presence of parasites, bacteria and viruses in human blood capable of providing analysis results within minutes was demonstrated.
Significance and Impact of the Study
There is an ongoing need for clinical diagnostics to adapt to newly emerging agents and to screen simultaneously for multiple infectious agents. A laser‐based approach can achieve sensitive, multiplex detection with minimal sample preparation and provide rapid results (within minutes). These properties along with the flexibility to add new agent detection by simply adjusting the detection programming make it a promising tool for clinical diagnosis.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14222</identifier><identifier>PMID: 30767345</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>ABO Blood-Group System ; Bacteria ; blood ; Blood groups ; Diagnostic systems ; HIV ; Humans ; Identification methods ; Infectious diseases ; Laboratory tests ; laser ; Lasers ; LIBS ; Microbiological Techniques - methods ; Parasites ; Protozoa ; rapid ; Sample preparation ; Sepsis - diagnosis ; Sepsis - microbiology ; Sepsis - parasitology ; Sepsis - virology ; Staphylococcus aureus ; Trypanosoma cruzi ; Viruses</subject><ispartof>Journal of applied microbiology, 2019-05, Vol.126 (5), p.1606-1617</ispartof><rights>2019 The Society for Applied Microbiology</rights><rights>2019 The Society for Applied Microbiology.</rights><rights>Copyright © 2019 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4432-b7fc488dea62124f69807c7703b04eaff1173925d4b8f7297ef08b6cc3a25daf3</citedby><cites>FETCH-LOGICAL-c4432-b7fc488dea62124f69807c7703b04eaff1173925d4b8f7297ef08b6cc3a25daf3</cites><orcidid>0000-0001-8409-2501 ; 0000-0002-0685-0191</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30767345$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Multari, R.A.</creatorcontrib><creatorcontrib>Cremers, D.A.</creatorcontrib><creatorcontrib>Nelson, A.</creatorcontrib><creatorcontrib>Karimi, Z.</creatorcontrib><creatorcontrib>Young, S.</creatorcontrib><creatorcontrib>Fisher, C.</creatorcontrib><creatorcontrib>Duncan, R.</creatorcontrib><title>The use of laser‐based diagnostics for the rapid identification of infectious agents in human blood</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
To investigate the use of a laser‐based method of detection as a potential diagnostic test for the rapid identification of infectious agents in human blood.
Methods and Results
In this study, the successful differentiation of blood spiked with viruses, bacteria or protozoan parasites to clinically relevant levels is demonstrated using six blood types (O+, O−, AB+, A+, A−, B+) using blood from different individuals with blood samples prepared in two different laboratories. Experiments were performed using various compositions of filters, experimental set‐ups and experimental parameters for spectral capture.
Conclusions
The potential for developing a laser‐based diagnostic instrument to detect the presence of parasites, bacteria and viruses in human blood capable of providing analysis results within minutes was demonstrated.
Significance and Impact of the Study
There is an ongoing need for clinical diagnostics to adapt to newly emerging agents and to screen simultaneously for multiple infectious agents. A laser‐based approach can achieve sensitive, multiplex detection with minimal sample preparation and provide rapid results (within minutes). These properties along with the flexibility to add new agent detection by simply adjusting the detection programming make it a promising tool for clinical diagnosis.</description><subject>ABO Blood-Group System</subject><subject>Bacteria</subject><subject>blood</subject><subject>Blood groups</subject><subject>Diagnostic systems</subject><subject>HIV</subject><subject>Humans</subject><subject>Identification methods</subject><subject>Infectious diseases</subject><subject>Laboratory tests</subject><subject>laser</subject><subject>Lasers</subject><subject>LIBS</subject><subject>Microbiological Techniques - methods</subject><subject>Parasites</subject><subject>Protozoa</subject><subject>rapid</subject><subject>Sample preparation</subject><subject>Sepsis - diagnosis</subject><subject>Sepsis - microbiology</subject><subject>Sepsis - parasitology</subject><subject>Sepsis - virology</subject><subject>Staphylococcus aureus</subject><subject>Trypanosoma cruzi</subject><subject>Viruses</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kc1O3DAUha2KqsCURV-gssQGFgH_xU42lRCCAgJ1A2vLcewZj5J4aiet2PEIPCNP0ssMoLYS3lz73s9H5-og9IWSIwrneGn6IyoYYx_QDuWyLJhUbGt9F0VJFNtGuzkvCaGclPIT2uZEScVFuYPc7cLhKTscPe5Mdunp4bGB2uI2mPkQ8xhsxj4mPAKYzCq0OLRuGIMP1owhDs8_w-CdhceUsZnDMEMHL6beDLjpYmw_o4_edNntvdQZujs_uz29KK5_fL88PbkurBCcFY3yVlRV64xklAkv64ooqxThDRHOeE-p4jUrW9FUXrFaOU-qRlrLDTSN5zP0baO7mpretRasJNPpVQq9Sfc6mqD_nQxhoefxl5airIhkIHDwIpDiz8nlUfchW9d1ZnCwnWa0FmXNiawA3f8PXcYpDbCeZoxIQglEAtThhrIp5pycfzNDiX4OT0N4eh0esF__dv9GvqYFwPEG-B06d_--kr46udlI_gENBKV2</recordid><startdate>201905</startdate><enddate>201905</enddate><creator>Multari, R.A.</creator><creator>Cremers, D.A.</creator><creator>Nelson, A.</creator><creator>Karimi, Z.</creator><creator>Young, S.</creator><creator>Fisher, C.</creator><creator>Duncan, R.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8409-2501</orcidid><orcidid>https://orcid.org/0000-0002-0685-0191</orcidid></search><sort><creationdate>201905</creationdate><title>The use of laser‐based diagnostics for the rapid identification of infectious agents in human blood</title><author>Multari, R.A. ; Cremers, D.A. ; Nelson, A. ; Karimi, Z. ; Young, S. ; Fisher, C. ; Duncan, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4432-b7fc488dea62124f69807c7703b04eaff1173925d4b8f7297ef08b6cc3a25daf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>ABO Blood-Group System</topic><topic>Bacteria</topic><topic>blood</topic><topic>Blood groups</topic><topic>Diagnostic systems</topic><topic>HIV</topic><topic>Humans</topic><topic>Identification methods</topic><topic>Infectious diseases</topic><topic>Laboratory tests</topic><topic>laser</topic><topic>Lasers</topic><topic>LIBS</topic><topic>Microbiological Techniques - methods</topic><topic>Parasites</topic><topic>Protozoa</topic><topic>rapid</topic><topic>Sample preparation</topic><topic>Sepsis - diagnosis</topic><topic>Sepsis - microbiology</topic><topic>Sepsis - parasitology</topic><topic>Sepsis - virology</topic><topic>Staphylococcus aureus</topic><topic>Trypanosoma cruzi</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Multari, R.A.</creatorcontrib><creatorcontrib>Cremers, D.A.</creatorcontrib><creatorcontrib>Nelson, A.</creatorcontrib><creatorcontrib>Karimi, Z.</creatorcontrib><creatorcontrib>Young, S.</creatorcontrib><creatorcontrib>Fisher, C.</creatorcontrib><creatorcontrib>Duncan, R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Multari, R.A.</au><au>Cremers, D.A.</au><au>Nelson, A.</au><au>Karimi, Z.</au><au>Young, S.</au><au>Fisher, C.</au><au>Duncan, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The use of laser‐based diagnostics for the rapid identification of infectious agents in human blood</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2019-05</date><risdate>2019</risdate><volume>126</volume><issue>5</issue><spage>1606</spage><epage>1617</epage><pages>1606-1617</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
To investigate the use of a laser‐based method of detection as a potential diagnostic test for the rapid identification of infectious agents in human blood.
Methods and Results
In this study, the successful differentiation of blood spiked with viruses, bacteria or protozoan parasites to clinically relevant levels is demonstrated using six blood types (O+, O−, AB+, A+, A−, B+) using blood from different individuals with blood samples prepared in two different laboratories. Experiments were performed using various compositions of filters, experimental set‐ups and experimental parameters for spectral capture.
Conclusions
The potential for developing a laser‐based diagnostic instrument to detect the presence of parasites, bacteria and viruses in human blood capable of providing analysis results within minutes was demonstrated.
Significance and Impact of the Study
There is an ongoing need for clinical diagnostics to adapt to newly emerging agents and to screen simultaneously for multiple infectious agents. A laser‐based approach can achieve sensitive, multiplex detection with minimal sample preparation and provide rapid results (within minutes). These properties along with the flexibility to add new agent detection by simply adjusting the detection programming make it a promising tool for clinical diagnosis.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>30767345</pmid><doi>10.1111/jam.14222</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-8409-2501</orcidid><orcidid>https://orcid.org/0000-0002-0685-0191</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of applied microbiology, 2019-05, Vol.126 (5), p.1606-1617 |
| issn | 1364-5072 1365-2672 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6458062 |
| source | Alma/SFX Local Collection |
| subjects | ABO Blood-Group System Bacteria blood Blood groups Diagnostic systems HIV Humans Identification methods Infectious diseases Laboratory tests laser Lasers LIBS Microbiological Techniques - methods Parasites Protozoa rapid Sample preparation Sepsis - diagnosis Sepsis - microbiology Sepsis - parasitology Sepsis - virology Staphylococcus aureus Trypanosoma cruzi Viruses |
| title | The use of laser‐based diagnostics for the rapid identification of infectious agents in human blood |
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