Ty-6, a major begomovirus resistance gene on chromosome 10, is effective against Tomato yellow leaf curl virus and Tomato mottle virus

• Published in: Theoretical and applied genetics 2019-05, Vol.132 (5), p.1543-1554
• Main Authors: Gill, Upinder, Scott, John W., Shekasteband, Reza, Ogundiwin, Eben, Schuit, Cees, Francis, David M., Sim, Sung-Chur, Smith, Hugh, Hutton, Samuel F.
• Format: Article
• Language: English
• Subjects: Agriculture; Begomovirus; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome 10; Chromosome Mapping; Chromosomes; Chromosomes, Plant; Disease resistance; Disease Resistance - genetics; DNA viruses; Gene mapping; Genes; Genes, Plant; Genetic aspects; Inbreeding; Leaf-curl; Leaves; Life Sciences; Lycopersicon esculentum - genetics; Lycopersicon esculentum - virology; Original; Original Article; Plant Biochemistry; Plant breeding; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - virology; Plant Genetics and Genomics; Plant viruses; Quantitative Trait Loci; Single-nucleotide polymorphism; Solanaceae; Tomatoes; Yellow leaf
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-019-03298-0

Key message Ty - 6 is a major resistance gene on chromosome 10 of tomato that provides resistance against monopartite and bipartite begomoviruses and complements resistance conferred by the known Ty - 3 and ty - 5 genes. Resistance to monopartite and bipartite begomoviruses is an important breeding objective for cultivated tomato. Several begomovirus resistance genes have been introgressed from related Solanum species and are available for breeding purposes. In the present study, we mapped an additional locus, Ty - 6 , to chromosome 10 of tomato. Ty - 6 is effective against both monopartite Tomato yellow leaf curl virus (TYLCV) and bipartite Tomato mottle virus (ToMoV). Gene action is incomplete dominance, with an intermediate resistance response when Ty - 6 is heterozygous. Analysis of populations segregating for Ty - 6 along with Ty - 3 or ty - 5 indicates that the highest level of resistance against TYLCV is attained when Ty - 6 is combined with an additional resistance allele. Our results also demonstrate that ty - 5 is ineffective against ToMoV. Although multiple SNPs linked to Ty - 6 were identified and can be used for breeding purposes, none of these were consistently polymorphic between Ty - 6 and ty - 6 breeding lines. Further research is underway to generate resequencing data for several Ty - 6 inbred lines for the discovery of additional sequence polymorphisms that can be used for fine mapping and characterizing the Ty - 6 locus.