Remote imaging of single cell 3D morphology with ultrafast coherent phonons and their resonance harmonics

Cell morphological analysis has long been used in cell biology and physiology for abnormality identification, early cancer detection, and dynamic change analysis under specific environmental stresses. This work reports on the remote mapping of cell 3D morphology with an in-plane resolution limited b...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2019-04, Vol.9 (1), p.6409-6409, Article 6409
Main Authors: Liu, Liwang, Viel, Alexis, Le Saux, Guillaume, Plawinski, Laurent, Muggiolu, Giovanna, Barberet, Philippe, Pereira, Marco, Ayela, Cédric, Seznec, Hervé, Durrieu, Marie-Christine, Olive, Jean-Marc, Audoin, Bertrand
Format: Article
Language:English
Subjects:
631/1647/245
639/624/1111/1115
639/766/25/3927
639/766/930/2735
Acoustics
Atomic force microscopy
Bone cancer
Cell Line, Tumor
Cell morphology
Cell Shape
Chemical Sciences
Cytology
Environmental stress
Humanities and Social Sciences
Humans
Imaging, Three-Dimensional
Macrophages
Macrophages - pathology
Mapping
Material chemistry
Monocytes
Monocytes - pathology
Morphology
multidisciplinary
Optics
Optics and Photonics
Osteosarcoma
Osteosarcoma - diagnostic imaging
Osteosarcoma - pathology
Phonons
Polymers
Polymethyl Methacrylate - chemistry
Resonance
Sarcoma
Science
Science (multidisciplinary)
Single-Cell Analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cell morphological analysis has long been used in cell biology and physiology for abnormality identification, early cancer detection, and dynamic change analysis under specific environmental stresses. This work reports on the remote mapping of cell 3D morphology with an in-plane resolution limited by optics and an out-of-plane accuracy down to a tenth of the optical wavelength. For this, GHz coherent acoustic phonons and their resonance harmonics were tracked by means of an ultrafast opto-acoustic technique. After illustrating the measurement accuracy with cell-mimetic polymer films we map the 3D morphology of an entire osteosarcoma cell. The resulting image complies with the image obtained by standard atomic force microscopy, and both reveal very close roughness mean values. In addition, while scanning macrophages and monocytes, we demonstrate an enhanced contrast of thickness mapping by taking advantage of the detection of high-frequency resonance harmonics. Illustrations are given with the remote quantitative imaging of the nucleus thickness gradient of migrating monocyte cells.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-42718-5