Low-Field, Benchtop NMR Spectroscopy as a Potential Tool for Point-of-Care Diagnostics of Metabolic Conditions: Validation, Protocols and Computational Models

Novel sensing technologies for liquid biopsies offer promising prospects for the early detection of metabolic conditions through omics techniques. Indeed, high-field nuclear magnetic resonance (NMR) facilities are routinely used for metabolomics investigations on a range of biofluids in order to rap...

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Bibliographic Details
Published in:Biotech (Basel) 2018-12, Vol.8 (1), p.2
Main Authors: Percival, Benita C, Grootveld, Martin, Gibson, Miles, Osman, Yasan, Molinari, Marco, Jafari, Fereshteh, Sahota, Tarsem, Martin, Mark, Casanova, Federico, Mather, Melissa L, Edgar, Mark, Masania, Jinit, Wilson, Philippe B
Format: Article
Language:English
Subjects:
Automation
Computer applications
Diabetes mellitus
Experiments
Glucose
Magnetic resonance spectroscopy
Mathematical models
Medical research
Metabolism
Metabolites
Metabolomics
NMR
Nuclear magnetic resonance
Pharmacy
Urine
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Summary:Novel sensing technologies for liquid biopsies offer promising prospects for the early detection of metabolic conditions through omics techniques. Indeed, high-field nuclear magnetic resonance (NMR) facilities are routinely used for metabolomics investigations on a range of biofluids in order to rapidly recognise unusual metabolic patterns in patients suffering from a range of diseases. However, these techniques are restricted by the prohibitively large size and cost of such facilities, suggesting a possible role for smaller, low-field NMR instruments in biofluid analysis. Herein we describe selected biomolecule validation on a low-field benchtop NMR spectrometer (60 MHz), and present an associated protocol for the analysis of biofluids on compact NMR instruments. We successfully detect common markers of diabetic control at low-to-medium concentrations through optimised experiments, including α-glucose (≤2.8 mmol/L) and acetone (25 µmol/L), and additionally in readily accessible biofluids, particularly human urine. We present a combined protocol for the analysis of these biofluids with low-field NMR spectrometers for metabolomics applications, and offer a perspective on the future of this technique appealing to 'point-of-care' applications.
ISSN:2571-5135
2571-5135
2673-6284
DOI:10.3390/ht8010002