Genetic Modification of Mucor circinelloides to Construct Stearidonic Acid Producing Cell Factory

Stearidonic acid (SDA; 18:4, n-3) is the delta 15-desaturase product of gamma linolenic acid (GLA; 18:3, n-6) and delta 6-desaturase product of alpha linolenic acid (ALA; 18:3, n-3). Construction of engineered oleaginous microbes have been attracting significant interest in producing SDA because of...

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Bibliographic Details
Published in:International journal of molecular sciences 2019-04, Vol.20 (7), p.1683
Main Authors: Khan, Md Ahsanul Kabir, Yang, Junhuan, Hussain, Syed Ammar, Zhang, Huaiyuan, Garre, Victoriano, Song, Yuanda
Format: Article
Language:English
Subjects:
alpha-Linolenic Acid - metabolism
Cloning
Desaturase
Enzymes
Fatty Acid Desaturases - genetics
Fatty Acid Desaturases - metabolism
Fatty acids
Fatty Acids, Omega-3 - metabolism
Fungi
gamma-Linolenic Acid - metabolism
Genes
Genetic engineering
Genetic modification
Genomes
Lipids
Microorganisms
Mortierella - genetics
Mortierella - metabolism
Mucor - genetics
Mucor - metabolism
Nitrogen
Oral administration
Plasmids
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Summary:Stearidonic acid (SDA; 18:4, n-3) is the delta 15-desaturase product of gamma linolenic acid (GLA; 18:3, n-6) and delta 6-desaturase product of alpha linolenic acid (ALA; 18:3, n-3). Construction of engineered oleaginous microbes have been attracting significant interest in producing SDA because of its nutritional value and pharmaceutical applications. is a GLA producing filamentous fungus, which can be a useful tool to produce SDA. This study has, therefore, overexpressed the ( ) gene from in this fungus to construct a SDA-producing cell factory. To produce SDA in , the homologous overexpression of gene was analyzed. When the gene was overexpressed in CBS 277.49, up to 5.0% SDA was accumulated in this strain. According to current knowledge, this is the first study describing the construction of a SDA-producing cell factory by overexpression of gene in oleaginous fungus . A new scope for further research has been established by this work to improve SDA production in this fungus, specifically in its high lipid-producing strain, WJ11.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20071683