Connected partner-switches control the life style of Pseudomonas aeruginosa through RpoS regulation

Biofilm formation is a complex process resulting from the action of imbricated pathways in response to environmental cues. In this study, we showed that biofilm biogenesis in the opportunistic pathogen Pseudomonas aeruginosa depends on the availability of RpoS, the sigma factor regulating the genera...

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Bibliographic Details
Published in:Scientific reports 2019-04, Vol.9 (1), p.6496-6496, Article 6496
Main Authors: Bouillet, Sophie, Ba, Moly, Houot, Laetitia, Iobbi-Nivol, Chantal, Bordi, Christophe
Format: Article
Language:English
Subjects:
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631/326/46
82/111
96/63
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biochemistry, Molecular Biology
Biofilms
Gene Expression Regulation, Bacterial
Humanities and Social Sciences
Kinases
Life Sciences
Models, Genetic
multidisciplinary
Opportunist infection
Phosphorylation
Post-translation
Protein Binding
Pseudomonas aeruginosa
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - metabolism
Pseudomonas aeruginosa - physiology
Science
Science (multidisciplinary)
Sigma factor
Sigma Factor - genetics
Sigma Factor - metabolism
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Summary:Biofilm formation is a complex process resulting from the action of imbricated pathways in response to environmental cues. In this study, we showed that biofilm biogenesis in the opportunistic pathogen Pseudomonas aeruginosa depends on the availability of RpoS, the sigma factor regulating the general stress response in bacteria. Moreover, it was demonstrated that RpoS is post-translationally regulated by the HsbR-HsbA partner switching system as has been demonstrated for its CrsR-CrsA homolog in Shewanella oneidensis . Finally, it was established that HsbA, the anti-sigma factor antagonist, has a pivotal role depending on its phosphorylation state since it binds HsbR, the response regulator, when phosphorylated and FlgM, the anti-sigma factor of FliA, when non-phosphorylated. The phosphorylation state of HsbA thus drives the switch between the sessile and planktonic way of life of P . aeruginosa by driving the release or the sequestration of one or the other of these two sigma factors.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-42653-5