Silencing of carbonic anhydrase I enhances the malignant potential of exosomes secreted by prostatic tumour cells

We report results showing that the silencing of carbonic anhydrase I (siCA1) in prostatic (PC3) tumour cells has a significant impact on exosome formation. An increased diameter, concentration and diversity of the produced exosomes were noticed as a consequence of this knock‐down. The protein compos...

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Bibliographic Details
Published in:Journal of cellular and molecular medicine 2019-05, Vol.23 (5), p.3641-3655
Main Authors: Bánová Vulić, Radivojka, Zdurienčíková, Martina, Tyčiaková, Silvia, Benada, Oldřich, Dubrovčáková, Mária, Lakota, Ján, Škultéty, Ľudovít
Format: Article
Language:English
Subjects:
carbonic anhydrase I
Carbonic Anhydrase I - genetics
Carbonic Anhydrase I - metabolism
Cell Movement - genetics
Cell Proliferation - genetics
Energy Metabolism - genetics
exosomes
Exosomes - genetics
Exosomes - metabolism
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Humans
LC‐MS
Male
malignant potential
Original
PC-3 Cells
PC3 cells
Prostate cancer
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
RNA Interference
siCA1
siMock
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Summary:We report results showing that the silencing of carbonic anhydrase I (siCA1) in prostatic (PC3) tumour cells has a significant impact on exosome formation. An increased diameter, concentration and diversity of the produced exosomes were noticed as a consequence of this knock‐down. The protein composition of the exosomes' cargo was also altered. Liquid chromatography and mass spectrometry analyses identified 42 proteins significantly altered in PC3 siCA1 exosomes compared with controls. The affected proteins are mainly involved in metabolic processes, biogenesis, cell component organization and defense/immunity. Interestingly, almost all of them have been described as ‘enhancers' of tumour development through the promotion of cell proliferation, migration and invasion. Thus, our results indicate that the reduced expression of the CA1 protein enhances the malignant potential of PC3 cells.
ISSN:1582-1838
1582-4934
DOI:10.1111/jcmm.14265