Long noncoding RNA CAR10 promotes lung adenocarcinoma metastasis via miR-203/30/SNAI axis

• Published in: Oncogene 2019-04, Vol.38 (16), p.3061-3076
• Main Authors: Ge, Xiaolu, Li, Gui-yuan, Jiang, Lin, Jia, Liqing, Zhang, Zhezhe, Li, Xiaoling, Wang, Ranran, Zhou, Ming, Zhou, Yanhong, Zeng, Zhaoyang, Xiang, Juanjuan, Li, Zheng
• Format: Article
• Language: English
• Subjects: 13/1; 13/109; 13/44; 13/51; 13/89; 14/1; 14/19; 14/34; 14/35; 14/5; 14/63; 38/1; 38/22; 38/23; 38/39; 38/44; 38/47; 38/61; 38/89; 42/41; 42/44; 42/89; 59/5; 631/337/384; 631/67/1612/1350; 631/67/322; 64/60; 82/1; A549 Cells; Adenocarcinoma; Adenocarcinoma of Lung - genetics; Animals; Antisense RNA; Apoptosis; Cancer metastasis; Cell Biology; Cell Line; Cell Line, Tumor; Chromatin; Chromatin - genetics; Crizotinib; Development and progression; Epithelial-Mesenchymal Transition - genetics; Female; Gene expression; Gene Expression Regulation, Neoplastic - genetics; Genetic aspects; HEK293 Cells; HeLa Cells; Hep G2 Cells; Human Genetics; Humans; Internal Medicine; Lung cancer; Lung Neoplasms - genetics; MCF-7 Cells; Medical schools; Medicine; Medicine & Public Health; Melanoma, Experimental - genetics; Mesenchyme; Metastases; Metastasis; Mice, Inbred BALB C; Mice, Nude; MicroRNAs - genetics; Novels; Oncology; Prognosis; Ribonucleic acid; RNA; RNA, Long Noncoding - genetics; Snail Family Transcription Factors - genetics; Snail protein; Stem cells; Therapeutic applications; Tumors; Up-Regulation - genetics
• ISSN: 0950-9232; 1476-5594
• DOI: 10.1038/s41388-018-0645-x

Long noncoding RNAs (lncRNAs) play an important role in lung adenocarcinoma (LUAD) metastasis. Here, we found that lncRNA chromatin-associated RNA 10 (CAR10) was upregulated in the tumor tissue of patients with LUAD and enhanced tumor metastasis in vitro and in vivo. Mechanistically, CAR10 induced epithelial-to-mesenchymal transition (EMT) by directly binding with miR-30 and miR-203 and then regulating the expression of SNAI1 and SNAI2 . CAR10 overexpression was positively correlated with a poor prognosis in LUAD patients, whereas overexpression of both CAR10 and SNAI was correlated with even worse clinical outcomes. In conclusion, the CAR10/miR-30/203/ SNAI axis is a novel and potential therapeutic target for LUAD.