Biosynthesis of L-Ascorbic Acid and Conversion of Carbons 1 and 2 of L-Ascorbic Acid to Oxalic Acid Occurs within Individual Calcium Oxalate Crystal Idioblasts

• Published in: Plant physiology (Bethesda) 2001-02, Vol.125 (2), p.634-640
• Main Authors: Todd A. Kostman, Nathan M. Tarlyn, Loewus, Frank A., Franceschi, Vincent R.
• Format: Article
• Language: English
• Subjects: Araceae - metabolism; Ascorbic Acid - biosynthesis; Autoradiography; Biochemical Processes and Macromolecular Structures; Biological and medical sciences; Biosynthesis; Calcium; Calcium metabolism; Calcium Oxalate - metabolism; Carbon Radioisotopes; Cell biochemistry; Cell physiology; Crystals; Fundamental and applied biological sciences. Psychology; Mesophyll; Mesophyll cells; Mineral precipitation; Oxalates; Oxalic acid; Oxalic Acid - metabolism; Plant physiology and development; Plant Shoots - metabolism; Plants; Protoplasts; Protoplasts - metabolism; Radioisotope Dilution Technique
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.125.2.634

L-Ascorbic acid (AsA) and its metabolic precursors give rise to oxalic acid (OxA) found in calcium oxalate crystals in specialized crystal idioblast cells in plants; however, it is not known if AsA and OxA are synthesized within the crystal idioblast cell or transported in from surrounding mesophyll cells. Isolated developing crystal idioblasts from Pistia stratiotes were used to study the pathway of OxA biosynthesis and to determine if idioblasts contain the entire path and are essentially independent in OxA synthesis. Idioblasts were supplied with various 14C-labeled compounds and examined by micro-autoradiography for incorporation of 14C into calcium oxalate crystals. [14C]OxA gave heavy labeling of crystals, indicating the isolated idioblasts are functional in crystal formation. Incubation with [1-14C]AsA also gave heavy labeling of crystals, whereas [6-14C]AsA gave no labeling. Labeled precursors of AsA (L-[1-14C]galactose; D-[1-14C]mannose) also resulted in crystal labeling, as did the ascorbic acid analog, D-[1-14C]erythorbic acid. Intensity of labeling of isolated idioblasts followed the pattern OxA > AsA (erythorbic acid) > L-galactose > D-mannose. Our results demonstrate that P. stratiotes crystal idioblasts synthesize the OxA used for crystal formation, the OxA is derived from the number 1 and 2 carbons of AsA, and the proposed pathway of ascorbic acid synthesis via D-mannose and L-galactose is operational in individual P. stratiotes crystal idioblasts. These results are discussed with respect to fine control of calcium oxalate precipitation and the concept of crystal idioblasts as independent physiological compartments.