Wavelength agile multi-photon microscopy with a fiber amplified diode laser

Multi-photon microscopy is a powerful tool in biomolecular research. Less complex and more cost effective excitation light sources will make this technique accessible to a broader community. Semiconductor diode seeded fiber lasers have proven to be especially robust, low cost and easy to use. Howeve...

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Bibliographic Details
Published in:Biomedical optics express 2018-12, Vol.9 (12), p.6273-6282
Main Authors: Eibl, Matthias, Weng, Daniel, Hakert, Hubertus, Kolb, Jan Philip, Pfeiffer, Tom, Hundt, Jennifer E, Huber, Robert, Karpf, Sebastian
Format: Article
Language:English
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Summary:Multi-photon microscopy is a powerful tool in biomolecular research. Less complex and more cost effective excitation light sources will make this technique accessible to a broader community. Semiconductor diode seeded fiber lasers have proven to be especially robust, low cost and easy to use. However, their wavelength tuning range is often limited, so only a limited number of fluorophores can be accessed. Therefore, different approaches have been proposed to extend the spectral coverage of these lasers. Recently, we showed that four-wave mixing (FWM) assisted stimulated Raman scattering (SRS) can be harnessed to red-shift high power pulses from 1064 nm to a narrowband output at 1122 nm and 1186 nm and therefore extend the number of accessible fluorophores. In this contribution, we show the applicability of all three wavelengths for multi-photon microscopy and analyze the performance.
ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.9.006273