Establishment and adipocyte differentiation of polycystic ovary syndrome-derived induced pluripotent stem cells

Objective To establish a new biological cell model and approach to mimic abnormal lipid metabolism of polycystic ovary syndrome (PCOS) in vitro. Materials and methods Epithelial cells from PCOS patients were reprogrammed to pluripotency by retroviral transduction using defined factors. Morphology, g...

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Bibliographic Details
Published in:Cell proliferation 2016-06, Vol.49 (3), p.352-361
Main Authors: Yang, Sheng, Ding, Shufang, Jiang, Xianglong, Sun, Bolan, Xu, Qianhua
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipocytes - metabolism
Adipogenesis
Adult
Cell Differentiation
Cells, Cultured
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Female
Glucose - metabolism
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Lipid Metabolism
Original
Polycystic Ovary Syndrome - metabolism
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Summary:Objective To establish a new biological cell model and approach to mimic abnormal lipid metabolism of polycystic ovary syndrome (PCOS) in vitro. Materials and methods Epithelial cells from PCOS patients were reprogrammed to pluripotency by retroviral transduction using defined factors. Morphology, growth characteristics, karyotype, gene expression and differentiation in vitro and in vivo were detected by identification protocol of human embryonic stem cells (ESCs). PCOS‐induced pluripotent stem cells (iPSCs) were then induced to differentiate into adipocytes. Ability of the adipocytes for glucose consumption was compared with those from non‐PCOS‐iPSCs. Results iPSCs were successfully generated from PCOS patients' adult cells. Formed iPSC clones had the same characteristics of human ESCs. PCOS‐iPSCs were induced to differentiation into normal karyotype adipocytes. Compared to non‐PCOS‐iPSCs, PCOS‐iPSCs had more glucose consumption ability during adipocyte differentiation and development in vitro. Conclusions This protocol provides a new biological cell model and approach for studying pathogenesis of PCOS and discovering potential drugs to treat it.
ISSN:0960-7722
1365-2184
DOI:10.1111/cpr.12258