Avenanthramide C from germinated oats exhibits anti-allergic inflammatory effects in mast cells

• Published in: Scientific reports 2019-05, Vol.9 (1), p.6884-6884, Article 6884
• Main Authors: Dhakal, Hima, Yang, Eun-Ju, Lee, Soyoung, Kim, Min-Jong, Baek, Moon-Chang, Lee, Byungheon, Park, Pil-Hoon, Kwon, Taeg Kyu, Khang, Dongwoo, Song, Kyung-Sik, Kim, Sang-Hyun
• Format: Article
• Language: English
• Subjects: 13/21; 631/250/256/2516; 692/308/153; AKT protein; Allergic diseases; Anaphylaxis; Animals; Anti-Allergic Agents - pharmacology; Anti-Inflammatory Agents - pharmacology; Avena - chemistry; Avena - growth & development; Biological properties; Bone marrow; Calcium (intracellular); Cell Degranulation - drug effects; Cytokines; Cytokines - metabolism; Degranulation; Extravasation; Germination; Histamine; Humanities and Social Sciences; Hypersensitivity; Hypothermia; Immunoglobulin E; Inflammation; Interleukin 4; Lyn protein; Male; Mast cells; Mast Cells - drug effects; Mast Cells - immunology; Mice; Mice, Inbred ICR; multidisciplinary; Oral administration; ortho-Aminobenzoates - pharmacology; Ovalbumin; Passive cutaneous anaphylaxis; Peritoneum; Phospholipase C; Phosphorylation; Science; Science (multidisciplinary); Syk protein
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-43412-2

Mast cells play a crucial role in allergic diseases via the release of inflammatory mediators, particularly histamine and pro-inflammatory cytokines. Avenanthramide (Avn) C, a polyphenol found mainly in oats, is known to exhibit various biological properties. In this study, we aimed to evaluate the effectiveness of Avn C from germinated oats against mast cell-mediated allergic inflammation. For the in vitro study, RBL-2H3, mouse bone marrow-derived mast cells and rat peritoneal mast cells were used. Avn C (1–100 nM) inhibited the immunoglobulin (Ig)E-stimulated mast cells degranulation by suppressing phosphorylation of phosphoinositide 3-kinase and phospholipase Cγ1 and decreasing intracellular calcium levels. It inhibited IgE-stimulated secretion of inflammatory cytokines via suppression of FcεRI-mediated signaling proteins Lyn, Syk, Akt, and nuclear factor-κB. To verify the effects of Avn C in vivo , ovalbumin-induced active systemic anaphylaxis (ASA) and IgE-mediated passive cutaneous anaphylaxis (PCA) models were used. Oral administration of Avn C dose-dependently attenuated the ASA reactions, as evidenced by the inhibition of hypothermia and reduction of elevated serum histamine, IgE, and interleukin-4 levels. Avn C also inhibited the PCA reactions, such as ear swelling and plasma extravasation. Our results suggested that Avn C from germinated oats might be a possible therapeutic candidate for mast cell-mediated allergic inflammation.