Activities of starch synthetic enzymes and contents of endogenous hormones in waxy maize grains subjected to post-silking water deficit

• Published in: Scientific reports 2019-05, Vol.9 (1), p.7059-7059, Article 7059
• Main Authors: Yang, Huan, Gu, Xiaotian, Ding, Mengqiu, Lu, Weiping, Lu, Dalei
• Format: Article
• Language: English
• Subjects: 1,4-alpha-Glucan Branching Enzyme - metabolism; 631/449/2661/2146; 631/449/2679/2682; Abscisic acid; Abscisic Acid - metabolism; Acetic acid; Adenosine diphosphate; Corn; Dehydration; Drought; Droughts; Enzymes; Enzymes - metabolism; Glucosyltransferases - metabolism; Hormones; Humanities and Social Sciences; Indoleacetic acid; Indoleacetic Acids - metabolism; Moisture content; multidisciplinary; Plant growth; Plant Proteins - metabolism; Plant reproduction; Pollination; Science; Science (multidisciplinary); Seeds - growth & development; Seeds - metabolism; Soil moisture; Starch; Starch - metabolism; Starch synthase; Starch Synthase - metabolism; Starch-branching enzymes; Sucrose; Sucrose phosphate synthase; Sucrose synthase; Water deficit; Zea mays - metabolism; Zea mays - physiology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-43484-0

Rainfed maize in Southern China and frequently suffer water deficit at later plant growth periods. A pot trial in 2014–2015 was conducted to study the effects of drought stress (the relative soil moisture contents are 70–80% and 50–60% under control and water deficit conditions, respectively) after pollination on grain filling and starch accumulation, activities of starch synthetic enzymes, and contents of indole-3-acetic acid (IAA) and abscisic acid (ABA), with Suyunuo5 as test material. The grain fresh weight, volume, and dry weight were not affected by drought before 10 days after pollination but were restricted thereafter. The reduction at maturity was reduced by 33.3%, 40.0%, and 32.3% in 2014 and by 21.7%, 24.3%, and 18.3% in 2015. The grain filling rate was suppressed by water deficit, whereas grain moisture and starch content were slightly affected. The starch accumulation was decreased by 33.5% and 20.0% at maturity in 2014 and 2015, respectively. The activities of starch synthetic enzymes (sucrose phosphate synthase, sucrose synthase, ADP-glucose pyrophosphorylase, soluble starch synthase, and starch branching enzyme) were downregulated by post-silking drought. The ABA content was increased, whereas IAA content was decreased when plants suffered water deficit during grain filling. In conclusion, post-silking water deficit increased ABA content, decreased IAA content, and weakened the activities of starch synthetic enzymes, which suppressed grain development and ultimately reduced grain weight.