Classification and characterization of hemocytes from two Asian horseshoe crab species Tachypleus tridentatus and Carcinoscorpius rotundicauda

In present study, transmission electron microscopy and flow cytometry were utilized to investigate the classification, characterization and immune functions of hemocytes from horseshoe crab, Tachypleus tridentatus and Carcinoscorpius rotundicauda . Three types of hemocytes were distinguished respect...

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Bibliographic Details
Published in:Scientific reports 2019-05, Vol.9 (1), p.7095-7095, Article 7095
Main Authors: Wu, Fangli, Xie, Zhe, Yan, Mingyan, Li, Qiongzhen, Song, Jie, Hu, Menghong, Wang, Youji
Format: Article
Language:English
Subjects:
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Animals
Blood Cell Count
Carboxylesterase - analysis
Carcinoscorpius rotundicauda
Crustaceans
Esterase
Female
Flow Cytometry
Hemocytes
Hemocytes - classification
Hemocytes - immunology
Horseshoe Crabs - cytology
Humanities and Social Sciences
Lysosomes - metabolism
Male
Microscopy, Electron, Transmission
multidisciplinary
Phagocytosis
Reactive oxygen species
Reactive Oxygen Species - analysis
Science
Science (multidisciplinary)
Subpopulations
Tachypleus tridentatus
Transmission electron microscopy
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Summary:In present study, transmission electron microscopy and flow cytometry were utilized to investigate the classification, characterization and immune functions of hemocytes from horseshoe crab, Tachypleus tridentatus and Carcinoscorpius rotundicauda . Three types of hemocytes were distinguished respectively: the granular cell, the semi-granular cell and the hyaline cell by transmission electron microscopy, while three hemocyte subpopulations (Gate 1 cell, Gate 2 cell, Gate 3 cell) were classified by flow cytometry. Hyaline cell was the major cell type with the highest nuclear-cytoplasmic ratio and granular cell and semi-granular cell showed lower ratios. Immune parameters of hemocytes in horseshoe crabs were investigated by flow cytometry. Different hemocyte subpopulations respond for diverse functions. Lysosomal contents and hemocyte mortality in Gate 3 cell subpopulation were higher than that in other subpopulations, while reactive oxygen species, phagocytosis and non-specific esterase, in Gate 1 cell subpopulation, were higher than those in other subpopulations. The hemocyte types between the two species had no significant differences in staining or morphology.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-43630-8