Real-Time 3D High-Resolution Microscopy of Human Cells on the International Space Station

Here we report the successful first operation of FLUMIAS-DEA, a miniaturized high-resolution 3D fluorescence microscope on the International Space Station (ISS) by imaging two scientific samples in a temperature-constant system, one sample with fixed cells and one sample with living human cells. The...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2019-04, Vol.20 (8), p.2033
Main Authors: Thiel, Cora Sandra, Tauber, Svantje, Seebacher, Christian, Schropp, Martin, Uhl, Rainer, Lauber, Beatrice, Polzer, Jennifer, Neelam, Srujana, Zhang, Ye, Ullrich, Oliver
Format: Article
Language:English
Subjects:
Actin
Adaptation
Aircraft industry
Cellular structure
Cytoskeleton
Experiments
Feasibility studies
Fluorescence
Gene expression
Gravitation
Gravity
Ground stations
High resolution
Lasers
Macrophages
Microscopy
Nuclei
Nuclei (cytology)
Parabolic flight
Physical sciences
Signal transduction
Simultaneous signals
Space stations
Systems stability
Three dimensional imaging
Vimentin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Here we report the successful first operation of FLUMIAS-DEA, a miniaturized high-resolution 3D fluorescence microscope on the International Space Station (ISS) by imaging two scientific samples in a temperature-constant system, one sample with fixed cells and one sample with living human cells. The FLUMIAS-DEA microscope combines features of a high-resolution 3D fluorescence microscope based on structured illumination microscope (SIM) technology with hardware designs to meet the requirements of a space instrument. We successfully demonstrated that the FLUMIAS technology was able to acquire, transmit, and store high-resolution 3D fluorescence images from fixed and living cells, allowing quantitative and dynamic analysis of subcellular structures, e.g., the cytoskeleton. The capability of real-time analysis methods on ISS will dramatically extend our knowledge about the dynamics of cellular reactions and adaptations to the space environment, which is not only an option, but a requirement of evidence-based medical risk assessment, monitoring and countermeasure development for exploration class missions.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20082033