Real-Time 3D High-Resolution Microscopy of Human Cells on the International Space Station

Here we report the successful first operation of FLUMIAS-DEA, a miniaturized high-resolution 3D fluorescence microscope on the International Space Station (ISS) by imaging two scientific samples in a temperature-constant system, one sample with fixed cells and one sample with living human cells. The...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2019-04, Vol.20 (8), p.2033
Main Authors: Thiel, Cora Sandra, Tauber, Svantje, Seebacher, Christian, Schropp, Martin, Uhl, Rainer, Lauber, Beatrice, Polzer, Jennifer, Neelam, Srujana, Zhang, Ye, Ullrich, Oliver
Format: Article
Language:English
Subjects:
Actin
Adaptation
Aircraft industry
Cellular structure
Cytoskeleton
Experiments
Feasibility studies
Fluorescence
Gene expression
Gravitation
Gravity
Ground stations
High resolution
Lasers
Macrophages
Microscopy
Nuclei
Nuclei (cytology)
Parabolic flight
Physical sciences
Signal transduction
Simultaneous signals
Space stations
Systems stability
Three dimensional imaging
Vimentin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c412t-5bf89d709dcd92cded7df3b50e0b45a306d16a2d108d59e5cd51deae754bc0973
cites cdi_FETCH-LOGICAL-c412t-5bf89d709dcd92cded7df3b50e0b45a306d16a2d108d59e5cd51deae754bc0973
container_end_page
container_issue 8
container_start_page 2033
container_title International journal of molecular sciences
container_volume 20
creator Thiel, Cora Sandra
Tauber, Svantje
Seebacher, Christian
Schropp, Martin
Uhl, Rainer
Lauber, Beatrice
Polzer, Jennifer
Neelam, Srujana
Zhang, Ye
Ullrich, Oliver
description Here we report the successful first operation of FLUMIAS-DEA, a miniaturized high-resolution 3D fluorescence microscope on the International Space Station (ISS) by imaging two scientific samples in a temperature-constant system, one sample with fixed cells and one sample with living human cells. The FLUMIAS-DEA microscope combines features of a high-resolution 3D fluorescence microscope based on structured illumination microscope (SIM) technology with hardware designs to meet the requirements of a space instrument. We successfully demonstrated that the FLUMIAS technology was able to acquire, transmit, and store high-resolution 3D fluorescence images from fixed and living cells, allowing quantitative and dynamic analysis of subcellular structures, e.g., the cytoskeleton. The capability of real-time analysis methods on ISS will dramatically extend our knowledge about the dynamics of cellular reactions and adaptations to the space environment, which is not only an option, but a requirement of evidence-based medical risk assessment, monitoring and countermeasure development for exploration class missions.
doi_str_mv 10.3390/ijms20082033
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6514950</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2332333367</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-5bf89d709dcd92cded7df3b50e0b45a306d16a2d108d59e5cd51deae754bc0973</originalsourceid><addsrcrecordid>eNpdkcFP2zAUxq0JNBjbbefJEhcOCzzbcVJfkFA3ViQQEu0OO1mO_dK6SuISJ5P47-cCqwq-2E_fT5_e54-QrwzOhVBw4ddt5AATDkJ8IMcs5zwDKMqDvfcR-RTjGoALLtVHciQY8JIV7Jj8eUDTZAvfIhU_6MwvV9kDxtCMgw8dvfO2D9GGzRMNNZ2NrenoFJsm0iQOK6Q33YB9Z7awaeh8YyzS-fA8fyaHtWkifnm9T8jv65-L6Sy7vf91M726zWzO-JDJqp4oV4Jy1iluHbrS1aKSgFDl0ggoHCsMdwwmTiqU1knm0GAp88qCKsUJuXzx3YxVi85iN_Sm0Zvet6Z_0sF4_Vbp_Eovw19dSJYrCcng7NWgD48jxkG3PtqU0nQYxqg5ZwVXvBA8oafv0HUYU_4mUSLp6RTbjb6_UNvPiz3Wu2UY6G1ner-zhH_bD7CD_5ck_gFY05Lk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2332333367</pqid></control><display><type>article</type><title>Real-Time 3D High-Resolution Microscopy of Human Cells on the International Space Station</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Thiel, Cora Sandra ; Tauber, Svantje ; Seebacher, Christian ; Schropp, Martin ; Uhl, Rainer ; Lauber, Beatrice ; Polzer, Jennifer ; Neelam, Srujana ; Zhang, Ye ; Ullrich, Oliver</creator><creatorcontrib>Thiel, Cora Sandra ; Tauber, Svantje ; Seebacher, Christian ; Schropp, Martin ; Uhl, Rainer ; Lauber, Beatrice ; Polzer, Jennifer ; Neelam, Srujana ; Zhang, Ye ; Ullrich, Oliver</creatorcontrib><description>Here we report the successful first operation of FLUMIAS-DEA, a miniaturized high-resolution 3D fluorescence microscope on the International Space Station (ISS) by imaging two scientific samples in a temperature-constant system, one sample with fixed cells and one sample with living human cells. The FLUMIAS-DEA microscope combines features of a high-resolution 3D fluorescence microscope based on structured illumination microscope (SIM) technology with hardware designs to meet the requirements of a space instrument. We successfully demonstrated that the FLUMIAS technology was able to acquire, transmit, and store high-resolution 3D fluorescence images from fixed and living cells, allowing quantitative and dynamic analysis of subcellular structures, e.g., the cytoskeleton. The capability of real-time analysis methods on ISS will dramatically extend our knowledge about the dynamics of cellular reactions and adaptations to the space environment, which is not only an option, but a requirement of evidence-based medical risk assessment, monitoring and countermeasure development for exploration class missions.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms20082033</identifier><identifier>PMID: 31027161</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Actin ; Adaptation ; Aircraft industry ; Cellular structure ; Cytoskeleton ; Experiments ; Feasibility studies ; Fluorescence ; Gene expression ; Gravitation ; Gravity ; Ground stations ; High resolution ; Lasers ; Macrophages ; Microscopy ; Nuclei ; Nuclei (cytology) ; Parabolic flight ; Physical sciences ; Signal transduction ; Simultaneous signals ; Space stations ; Systems stability ; Three dimensional imaging ; Vimentin</subject><ispartof>International journal of molecular sciences, 2019-04, Vol.20 (8), p.2033</ispartof><rights>2019. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-5bf89d709dcd92cded7df3b50e0b45a306d16a2d108d59e5cd51deae754bc0973</citedby><cites>FETCH-LOGICAL-c412t-5bf89d709dcd92cded7df3b50e0b45a306d16a2d108d59e5cd51deae754bc0973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2332333367/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2332333367?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25731,27901,27902,36989,36990,44566,53766,53768,74869</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31027161$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thiel, Cora Sandra</creatorcontrib><creatorcontrib>Tauber, Svantje</creatorcontrib><creatorcontrib>Seebacher, Christian</creatorcontrib><creatorcontrib>Schropp, Martin</creatorcontrib><creatorcontrib>Uhl, Rainer</creatorcontrib><creatorcontrib>Lauber, Beatrice</creatorcontrib><creatorcontrib>Polzer, Jennifer</creatorcontrib><creatorcontrib>Neelam, Srujana</creatorcontrib><creatorcontrib>Zhang, Ye</creatorcontrib><creatorcontrib>Ullrich, Oliver</creatorcontrib><title>Real-Time 3D High-Resolution Microscopy of Human Cells on the International Space Station</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Here we report the successful first operation of FLUMIAS-DEA, a miniaturized high-resolution 3D fluorescence microscope on the International Space Station (ISS) by imaging two scientific samples in a temperature-constant system, one sample with fixed cells and one sample with living human cells. The FLUMIAS-DEA microscope combines features of a high-resolution 3D fluorescence microscope based on structured illumination microscope (SIM) technology with hardware designs to meet the requirements of a space instrument. We successfully demonstrated that the FLUMIAS technology was able to acquire, transmit, and store high-resolution 3D fluorescence images from fixed and living cells, allowing quantitative and dynamic analysis of subcellular structures, e.g., the cytoskeleton. The capability of real-time analysis methods on ISS will dramatically extend our knowledge about the dynamics of cellular reactions and adaptations to the space environment, which is not only an option, but a requirement of evidence-based medical risk assessment, monitoring and countermeasure development for exploration class missions.</description><subject>Actin</subject><subject>Adaptation</subject><subject>Aircraft industry</subject><subject>Cellular structure</subject><subject>Cytoskeleton</subject><subject>Experiments</subject><subject>Feasibility studies</subject><subject>Fluorescence</subject><subject>Gene expression</subject><subject>Gravitation</subject><subject>Gravity</subject><subject>Ground stations</subject><subject>High resolution</subject><subject>Lasers</subject><subject>Macrophages</subject><subject>Microscopy</subject><subject>Nuclei</subject><subject>Nuclei (cytology)</subject><subject>Parabolic flight</subject><subject>Physical sciences</subject><subject>Signal transduction</subject><subject>Simultaneous signals</subject><subject>Space stations</subject><subject>Systems stability</subject><subject>Three dimensional imaging</subject><subject>Vimentin</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNpdkcFP2zAUxq0JNBjbbefJEhcOCzzbcVJfkFA3ViQQEu0OO1mO_dK6SuISJ5P47-cCqwq-2E_fT5_e54-QrwzOhVBw4ddt5AATDkJ8IMcs5zwDKMqDvfcR-RTjGoALLtVHciQY8JIV7Jj8eUDTZAvfIhU_6MwvV9kDxtCMgw8dvfO2D9GGzRMNNZ2NrenoFJsm0iQOK6Q33YB9Z7awaeh8YyzS-fA8fyaHtWkifnm9T8jv65-L6Sy7vf91M726zWzO-JDJqp4oV4Jy1iluHbrS1aKSgFDl0ggoHCsMdwwmTiqU1knm0GAp88qCKsUJuXzx3YxVi85iN_Sm0Zvet6Z_0sF4_Vbp_Eovw19dSJYrCcng7NWgD48jxkG3PtqU0nQYxqg5ZwVXvBA8oafv0HUYU_4mUSLp6RTbjb6_UNvPiz3Wu2UY6G1ner-zhH_bD7CD_5ck_gFY05Lk</recordid><startdate>20190425</startdate><enddate>20190425</enddate><creator>Thiel, Cora Sandra</creator><creator>Tauber, Svantje</creator><creator>Seebacher, Christian</creator><creator>Schropp, Martin</creator><creator>Uhl, Rainer</creator><creator>Lauber, Beatrice</creator><creator>Polzer, Jennifer</creator><creator>Neelam, Srujana</creator><creator>Zhang, Ye</creator><creator>Ullrich, Oliver</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190425</creationdate><title>Real-Time 3D High-Resolution Microscopy of Human Cells on the International Space Station</title><author>Thiel, Cora Sandra ; Tauber, Svantje ; Seebacher, Christian ; Schropp, Martin ; Uhl, Rainer ; Lauber, Beatrice ; Polzer, Jennifer ; Neelam, Srujana ; Zhang, Ye ; Ullrich, Oliver</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-5bf89d709dcd92cded7df3b50e0b45a306d16a2d108d59e5cd51deae754bc0973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Actin</topic><topic>Adaptation</topic><topic>Aircraft industry</topic><topic>Cellular structure</topic><topic>Cytoskeleton</topic><topic>Experiments</topic><topic>Feasibility studies</topic><topic>Fluorescence</topic><topic>Gene expression</topic><topic>Gravitation</topic><topic>Gravity</topic><topic>Ground stations</topic><topic>High resolution</topic><topic>Lasers</topic><topic>Macrophages</topic><topic>Microscopy</topic><topic>Nuclei</topic><topic>Nuclei (cytology)</topic><topic>Parabolic flight</topic><topic>Physical sciences</topic><topic>Signal transduction</topic><topic>Simultaneous signals</topic><topic>Space stations</topic><topic>Systems stability</topic><topic>Three dimensional imaging</topic><topic>Vimentin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thiel, Cora Sandra</creatorcontrib><creatorcontrib>Tauber, Svantje</creatorcontrib><creatorcontrib>Seebacher, Christian</creatorcontrib><creatorcontrib>Schropp, Martin</creatorcontrib><creatorcontrib>Uhl, Rainer</creatorcontrib><creatorcontrib>Lauber, Beatrice</creatorcontrib><creatorcontrib>Polzer, Jennifer</creatorcontrib><creatorcontrib>Neelam, Srujana</creatorcontrib><creatorcontrib>Zhang, Ye</creatorcontrib><creatorcontrib>Ullrich, Oliver</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep (ProQuest)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thiel, Cora Sandra</au><au>Tauber, Svantje</au><au>Seebacher, Christian</au><au>Schropp, Martin</au><au>Uhl, Rainer</au><au>Lauber, Beatrice</au><au>Polzer, Jennifer</au><au>Neelam, Srujana</au><au>Zhang, Ye</au><au>Ullrich, Oliver</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Real-Time 3D High-Resolution Microscopy of Human Cells on the International Space Station</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2019-04-25</date><risdate>2019</risdate><volume>20</volume><issue>8</issue><spage>2033</spage><pages>2033-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Here we report the successful first operation of FLUMIAS-DEA, a miniaturized high-resolution 3D fluorescence microscope on the International Space Station (ISS) by imaging two scientific samples in a temperature-constant system, one sample with fixed cells and one sample with living human cells. The FLUMIAS-DEA microscope combines features of a high-resolution 3D fluorescence microscope based on structured illumination microscope (SIM) technology with hardware designs to meet the requirements of a space instrument. We successfully demonstrated that the FLUMIAS technology was able to acquire, transmit, and store high-resolution 3D fluorescence images from fixed and living cells, allowing quantitative and dynamic analysis of subcellular structures, e.g., the cytoskeleton. The capability of real-time analysis methods on ISS will dramatically extend our knowledge about the dynamics of cellular reactions and adaptations to the space environment, which is not only an option, but a requirement of evidence-based medical risk assessment, monitoring and countermeasure development for exploration class missions.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>31027161</pmid><doi>10.3390/ijms20082033</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1422-0067
ispartof International journal of molecular sciences, 2019-04, Vol.20 (8), p.2033
issn 1422-0067
1661-6596
1422-0067
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6514950
source Publicly Available Content Database; PubMed Central
subjects Actin
Adaptation
Aircraft industry
Cellular structure
Cytoskeleton
Experiments
Feasibility studies
Fluorescence
Gene expression
Gravitation
Gravity
Ground stations
High resolution
Lasers
Macrophages
Microscopy
Nuclei
Nuclei (cytology)
Parabolic flight
Physical sciences
Signal transduction
Simultaneous signals
Space stations
Systems stability
Three dimensional imaging
Vimentin
title Real-Time 3D High-Resolution Microscopy of Human Cells on the International Space Station
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T23%3A57%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Real-Time%203D%20High-Resolution%20Microscopy%20of%20Human%20Cells%20on%20the%20International%20Space%20Station&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Thiel,%20Cora%20Sandra&rft.date=2019-04-25&rft.volume=20&rft.issue=8&rft.spage=2033&rft.pages=2033-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms20082033&rft_dat=%3Cproquest_pubme%3E2332333367%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c412t-5bf89d709dcd92cded7df3b50e0b45a306d16a2d108d59e5cd51deae754bc0973%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2332333367&rft_id=info:pmid/31027161&rfr_iscdi=true