Genome-wide association study of seed protein, oil and amino acid contents in soybean from maturity groups I to IV

• Published in: Theoretical and applied genetics 2019-06, Vol.132 (6), p.1639-1659
• Main Authors: Lee, Sungwoo, Van, Kyujung, Sung, Mikyung, Nelson, Randall, LaMantia, Jonathan, McHale, Leah K., Mian, M. A. Rouf
• Format: Article
• Language: English
• Subjects: Agriculture; Amino acids; Amino Acids - metabolism; Analysis; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome 10; Chromosome 20; Chromosome 5; Chromosome Mapping; Chromosomes; Chromosomes, Plant - genetics; Chromosomes, Plant - metabolism; Cysteine; Essential oils; Food industry; Genome, Plant; Genome-wide association studies; Genome-Wide Association Study; Genomes; Genomics; Geographical distribution; Glycine max; Glycine max - genetics; Glycine max - metabolism; Haplotypes; Life Sciences; Linkage Disequilibrium; Lysine; Maturity; Methionine; Nutrient content; Original; Original Article; Phenotype; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Proteins - genetics; Plant Proteins - metabolism; Polymorphism, Single Nucleotide; Proteins; Quantitative genetics; Quantitative Trait Loci; Seeds - metabolism; Soybean; Soybean Oil - metabolism; Soybeans; Threonine
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-019-03304-5

Key message Genomic regions associated with seed protein, oil and amino acid contents were identified by genome-wide association analyses. Geographic distributions of haplotypes indicate scope of improvement of these traits. Soybean [ Glycine max (L.) Merr.] protein and oil are used worldwide in feed, food and industrial materials. Increasing seed protein and oil contents is important; however, protein content is generally negatively correlated with oil content. We conducted a genome-wide association study using phenotypic data collected from five environments for 621 accessions in maturity groups I–IV and 34,014 markers to identify quantitative trait loci (QTL) for seed content of protein, oil and several essential amino acids. Three and five genomic regions were associated with seed protein and oil contents, respectively. One, three, one and four genomic regions were associated with cysteine, methionine, lysine and threonine content (g kg −1 crude protein), respectively. As previously shown, QTL on chromosomes 15 and 20 were associated with seed protein and oil contents, with both exhibiting opposite effects on the two traits, and the chromosome 20 QTL having the most significant effect. A multi-trait mixed model identified trait-specific QTL. A QTL on chromosome 5 increased oil with no effect on protein content, and a QTL on chromosome 10 increased protein content with little effect on oil content. The chromosome 10 QTL co-localized with maturity gene E2 / GmGIa . Identification of trait-specific QTL indicates feasibility to reduce the negative correlation between protein and oil contents. Haplotype blocks were defined at the QTL identified on chromosomes 5, 10, 15 and 20. Frequencies of positive effect haplotypes varied across maturity groups and geographic regions, providing guidance on which alleles have potential to contribute to soybean improvement for specific regions.