High fidelity fibre-based physiological sensing deep in tissue

Physiological sensing deep in tissue remains a clinical challenge. Here a flexible miniaturised sensing optrode providing a platform to perform minimally invasive in vivo in situ measurements is reported. Silica microspheres covalently coupled with a high density of ratiometrically configured fluoro...

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Bibliographic Details
Published in:Scientific reports 2019-05, Vol.9 (1), p.7713-7713, Article 7713
Main Authors: Choudhary, Tushar R., Tanner, Michael G., Megia-Fernandez, Alicia, Harrington, Kerrianne, Wood, Harry A., Marshall, Adam, Zhu, Patricia, Chankeshwara, Sunay V., Choudhury, Debaditya, Monro, Graham, Ucuncu, Muhammed, Yu, Fei, Duncan, Rory R., Thomson, Robert R., Dhaliwal, Kevin, Bradley, Mark
Format: Article
Language:English
Subjects:
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Alveoli
Animals
Bronchoscopy
Female
Fiber Optic Technology - methods
Fluoresceins - analysis
Fluorescent Dyes - analysis
Fluorophores
Humanities and Social Sciences
Hydrogen-Ion Concentration
In situ measurement
Microscopy, Electron, Scanning
Microspheres
Miniaturization
multidisciplinary
Optical Fibers
Oxygen
pH effects
Physiology
Pulmonary Alveoli - diagnostic imaging
Rhodamines - analysis
Science
Science (multidisciplinary)
Sheep
Silica
Silicon Dioxide
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Summary:Physiological sensing deep in tissue remains a clinical challenge. Here a flexible miniaturised sensing optrode providing a platform to perform minimally invasive in vivo in situ measurements is reported. Silica microspheres covalently coupled with a high density of ratiometrically configured fluorophores were deposited into etched pits on the distal end of a 150 µm diameter multicore optical fibre. With this platform, photonic measurements of pH and oxygen concentration with high precision in the distal alveolar space of the lung are reported. We demonstrated the phenomenon that high-density deposition of carboxyfluorescein covalently coupled to silica microspheres shows an inverse shift in fluorescence in response to varying pH. This platform delivered fast and accurate measurements (±0.02 pH units and ±0.6 mg/L of oxygen), near instantaneous response time and a flexible architecture for addition of multiple sensors.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-44077-7