Glyceryl trinitrate blocks staphyloxanthin and biofilm formation in Staphylococcus aureus

Background: Staphylococcus aureus is an important nosocomial bacterium that is responsible for a number of infections that may be fatal. The treatment of such infections is limited by emergence of antibiotic resistance. Targeting virulence of Staphylococcus aureus may provide an alternative option t...

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Bibliographic Details
Published in:African health sciences 2019-03, Vol.19 (1), p.1376-1384
Main Authors: Abbas, Hisham A, Elsherbini, Ahmed M, Shaldam, Moutaz A
Format: Article
Language:English
Subjects:
biofilm
Biofilms - drug effects
Drug Resistance, Microbial
Glyceryl trinitrate
Humans
Molecular Docking Simulation
Nitroglycerin - pharmacology
Oxidative Stress - drug effects
Staphylococcal Infections - microbiology
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - pathogenicity
staphyloxanthin
Virulence - drug effects
Virulence Factors
Xanthophylls - biosynthesis
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Summary:Background: Staphylococcus aureus is an important nosocomial bacterium that is responsible for a number of infections that may be fatal. The treatment of such infections is limited by emergence of antibiotic resistance. Targeting virulence of Staphylococcus aureus may provide an alternative option to antibiotic that may bypass the emergence of resistant strains due to lack of stress on viability. Objectives: Investigation of the ability of glyceryl trinitrate (GTN) to inhibit staphyloxanthin, biofilm and tolerance to oxidative stress. Methods: The disk sensitivity method was used to detect the methicillin resistance of Staphylococcus aureus. The effect of sub-inhibitory concentration of GTN on biofilm formation, staphyloxanthin production and tolerance to oxidative stress was evaluated. Molecular docking study was used to investigate the ability of GTN to bind to dehydrosqualene synthase enzyme. Results: GTN showed a significant inhibition of biofilm, staphyloxanthin and tolerance to oxidative stress. In the molecular docking study, it was found that GTN could bind to dehydrosqualene synthase enzyme by hydrogen bonding,electrostatic interaction and pi-cation interaction. Conclusion: The present study revealed the ability of GTN to serve as a potential anti-virulence candidate for attenuation of S. aureus pathogenicity. DOI: https://dx.doi.org/10.4314/ahs.v19i1.10 Cite as: Abbas HA, Elsherbini AM, MA S. Glyceryl trinitrate blocks staphyloxanthin and biofilm formation in Staphylococcus aureus. Afri Health Sci. 2019;19(1). 1376-1384. https://dx.doi.org/10.4314/ahs.v19i1.10
ISSN:1680-6905
1729-0503
1680-6905
DOI:10.4314/ahs.v19i1.10