Vaginal Microbiota Composition Correlates Between Pap Smear Microscopy and Next Generation Sequencing and Associates to Socioeconomic Status

Recent research on vaginal microbiota relies on high throughput sequencing while microscopic methods have a long history in clinical use. We investigated the correspondence between microscopic findings of Pap smears and the vaginal microbiota composition determined by next generation sequencing amon...

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Bibliographic Details
Published in:Scientific reports 2019-05, Vol.9 (1), p.7750-7750, Article 7750
Main Authors: Virtanen, Seppo, Rantsi, Tiina, Virtanen, Anni, Kervinen, Kaisa, Nieminen, Pekka, Kalliala, Ilkka, Salonen, Anne
Format: Article
Language:English
Subjects:
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Adult
Bacteria - genetics
Cytolysis
Economics
Epithelial cells
Epithelial Cells - microbiology
Female
Finland
Gardnerella vaginalis - genetics
High-Throughput Nucleotide Sequencing - methods
Humanities and Social Sciences
Humans
Lactobacillus - genetics
Microbiota
Microbiota - genetics
Microscopy
Microscopy - methods
multidisciplinary
Next-generation sequencing
Pap smear
Papanicolaou Test - methods
Reproducibility of Results
RNA, Ribosomal, 16S - genetics
Science
Science (multidisciplinary)
Social Class
Socioeconomic factors
Socioeconomic status
Socioeconomics
Vagina
Vagina - microbiology
Vaginal Smears - methods
Vaginosis
Vaginosis, Bacterial - diagnosis
Vaginosis, Bacterial - genetics
Vaginosis, Bacterial - microbiology
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Summary:Recent research on vaginal microbiota relies on high throughput sequencing while microscopic methods have a long history in clinical use. We investigated the correspondence between microscopic findings of Pap smears and the vaginal microbiota composition determined by next generation sequencing among 50 asymptomatic women. Both methods produced coherent results regarding the distinction between Lactobacillus -dominant versus mixed microbiota, reassuring gynaecologists for the use of Pap smear or wet mount microscopy for rapid evaluation of vaginal bacteria as part of diagnosis. Cytologic findings identified women with bacterial vaginosis and revealed that cytolysis of vaginal epithelial cells is associated to Lactobacillus crispatus -dominated microbiota. Education and socio-economic status were associated to the vaginal microbiota variation. Our results highlight the importance of including socio-economic status as a co-factor in future vaginal microbiota studies.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-44157-8