microRNA and Other Small RNA Sequence Profiling across Six Tissues of Chinese Forest Musk Deer (Moschus berezovskii)

• Published in: BioMed research international 2019-01, Vol.2019 (2019), p.1-9
• Main Authors: Li, Diyan, Zeng, Dejun, Lei, Meiyan, Mishra, Shailendra Kumar, Xu, Zhongxian, Zhang, Pu, Jie, Hang, Zhao, Guijun
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Cancer; Cell adhesion & migration; China; Deer; Deer - genetics; Deer - metabolism; Developmental biology; Foot-and-mouth disease; Forests; Gene expression; Gene Expression Profiling; Gene Expression Regulation - physiology; Genes; Genetic diversity; Heart; High-Throughput Nucleotide Sequencing; Hypothesis testing; Inbreeding; Liver; Lungs; Medical research; MicroRNA; MicroRNAs; MicroRNAs - biosynthesis; MicroRNAs - genetics; miRNA; Moschidae; Moschus berezovskii; Muscles; Nucleotide sequence; Organ Specificity - physiology; Prostate; Ribonucleic acid; RNA; Spleen; Tissues
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2019/4370704

The Chinese forest musk deer (Moschus berezovskii) is an economically important species distributed throughout southwest China and northern Vietnam. Occurrence and development of disease are aggravated by inbreeding and genetic diversity declines in captive musk deer populations. Deep transcriptomics investigation may provide a promising way to improve genetic health of captive and wild FMD population. MicroRNAs (miRNAs), which regulate gene expression by targeting and suppressing of mRNAs, play an important role in physiology and organism development control. In this study, RNA-seq technology was adopted to characterize the miRNA transcriptome signature among six tissues (heart, liver, spleen, lung, kidney, and muscle) in Chinese forest musk deer at two years of age. Deep sequencing generated a total of 103,261,451 (~87.87%) good quality small RNA reads; of them 6,622,520 were unique across all six tissues. A total of 2890 miRNAs were identified, among them 1129 were found to be expressed in all tissues. Moreover, coexpression of 20 miRNAs (>2000RPM) in all six tissues and top five highly expressed miRNAs in each tissue implied the crucial and particular function of them in FMD physiological processes. Our findings of forest musk deer miRNAs supplement the database of transcriptome information for this species and conduce to our understanding of forest musk deer biology.