Strain-Level Differentiation of Bacteria by Paper Spray Ionization Mass Spectrometry

Paper spray ionization mass spectrometry (PSI-MS) is a relatively new analytical technique allowing for rapid mass spectrometric analysis of biological samples with little or no sample preparation. The expeditious nature of the analysis and minimal requirement for sample preparation make PSI-MS a pr...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2019-04, Vol.91 (8), p.4964-4968
Main Authors: Chamberlain, Casey A, Rubio, Vanessa Y, Garrett, Timothy J
Format: Article
Language:English
Subjects:
Bacteria
Biological properties
Biological samples
Biomarkers
Chemistry
Differentiation
Intestine
Ionization
Ions
Lysates
Mass spectrometry
Mass Spectrometry - methods
Mass spectroscopy
Metabolites
Multivariate statistical analysis
Oxalobacter formigenes - chemistry
Oxalobacter formigenes - metabolism
Sample preparation
Scientific imaging
Species Specificity
Spectroscopy
Statistical analysis
Statistical methods
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Summary:Paper spray ionization mass spectrometry (PSI-MS) is a relatively new analytical technique allowing for rapid mass spectrometric analysis of biological samples with little or no sample preparation. The expeditious nature of the analysis and minimal requirement for sample preparation make PSI-MS a promising avenue for future clinical assays with one potential application in the identification of different types of bacteria. Although past PSI-MS studies have demonstrated the ability to distinguish between bacteria of different species and morphological classes, achieving within-species strain-level differentiation has never been performed. In this report, we demonstrate the first strain-level bacterial differentiation by PSI-MS with the mammalian intestinal bacterium Oxalobacter formigenes (Oxf). This novel application holds promising clinical significance as it could be used to differentiate between pathogenic bacteria and their harmless, commensal relatives, saving time and money in clinical diagnostics. Both whole cells and cell lysates of Oxf strains HC1 and OxWR were analyzed using the Prosolia Velox 360TM PSI source coupled to a Thermo Scientific Q Exactive high-resolution mass spectrometer with a rapid 30 s analytical method. Multivariate statistical analysis followed by examination of significant features provided for and confirmed differentiation between Oxf HC1 and OxWR. We report a panel of strain-exclusive metabolites that could serve as potential strain-indicating biomarkers.
ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.9b00330