Enhanced osteogenic proliferation and differentiation of human adipose-derived stem cells on a porous n-HA/PGS-M composite scaffold

• Published in: Scientific reports 2019-05, Vol.9 (1), p.7960-7960, Article 7960
• Main Authors: Wang, Yaozong, Sun, Naikun, Zhang, Yinlong, Zhao, Bin, Zhang, Zheyi, Zhou, Xu, Zhou, Yuanyuan, Liu, Hongyi, Zhang, Ying, Liu, Jianguo
• Format: Article
• Language: English
• Subjects: 13/100; 13/107; 38/77; 631/61/2320; 639/925/352/1061; Apatite; Biocompatibility; Biomarkers - metabolism; Calorimetry; Cbfa-1 protein; Cell Adhesion - drug effects; Cell Differentiation - drug effects; Cell proliferation; Cell Proliferation - drug effects; Cell Survival - drug effects; Collagen (type I); Collagen Type I - genetics; Collagen Type I - metabolism; Compressibility; Core Binding Factor Alpha 1 Subunit - genetics; Core Binding Factor Alpha 1 Subunit - metabolism; Decanoates - chemistry; Differential scanning calorimetry; Durapatite - chemistry; Durapatite - pharmacology; Gene Expression; Glycerol; Glycerol - analogs & derivatives; Glycerol - chemistry; Humanities and Social Sciences; Humans; Hydroxyapatite; Maleic Anhydrides - chemistry; Materials Testing; Mechanical properties; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - drug effects; Mesenchymal Stem Cells - metabolism; multidisciplinary; NMR; Nuclear magnetic resonance; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - metabolism; Osteocalcin - genetics; Osteocalcin - metabolism; Osteogenesis - drug effects; Osteogenesis - genetics; Polymers - chemistry; Pore size; Porosity; Primary Cell Culture; Scanning electron microscopy; Science; Science (multidisciplinary); Stem cells; Tissue engineering; Tissue Engineering - methods; Tissue Scaffolds; Transition temperatures
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-44478-8

This study explored the applicability, cellular efficacy, and osteogenic activities of porous nano-hydroxyapatite/Poly (glycerol sebacate)-grafted maleic anhydride (n-HA/PGS-g-M) composite scaffolds. Nuclear magnetic resonance (NMR) analyses indicated that approximately 43% of the hydroxide radicals in PGS were displaced by maleic anhydride. Resonance bands at 1036 cm −1 occurred in scaffolds containing nHA powders, and peak areas increased when n-HA weight increased in PGS-M-n-HA-0.4, PGS-M-n-HA-0.5, and PGS-M-n-HA-0.6 scaffolds. The n-HA/PGS-g-M composite scaffolds exhibited porous microstructure with average pore size of 150–300 µm in scanning electron microscopy (SEM) analysis. Differential scanning calorimetry (DSC) identified the glass transition temperature (Tg) as −25–30 °C, indicative of quality resilience. The modulus of compressibility increased when n-HA content increased. Interestingly, viability of human adipose-derived stem cells (hADSCs) in vitro and expression of the osteogenic related genes RUNX2 , OCN , and COL1A1 was enhanced in the n-HA/PGS-g-M composite scaffolds compared to those factors observed in PGS-g-M scaffolds. Finally, simulated body fluid (SBF) tests indicated more apatite deposits on the surface of n-HA/PGS-g-M scaffolds compared to PGS-g-M scaffolds. Overall, porous n-HA/PGS-g-M composite scaffolds possessed acceptable biocompatibility and mechanical properties, and they stimulated hADSC cell proliferation and differentiation. Given these qualities, the composite scaffolds have potential applications in bone tissue engineering.