A Thermostable Aspergillus fumigatus GH7 Endoglucanase Over-Expressed in Pichia pastoris Stimulates Lignocellulosic Biomass Hydrolysis

In the context of avoiding the use of non-renewable energy sources, employing lignocellulosic biomass for ethanol production remains a challenge. Cellulases play an important role in this scenario: they are some of the most important industrial enzymes that can hydrolyze lignocellulose. This study a...

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Bibliographic Details
Published in:International journal of molecular sciences 2019-05, Vol.20 (9), p.2261
Main Authors: Vianna Bernardi, Aline, Kimie Yonamine, Deborah, Akira Uyemura, Sergio, Magnani Dinamarco, Taisa
Format: Article
Language:English
Subjects:
Barley
Biofuels
Biomass
Carbohydrates
Cellulase
Cellulose
Cloning
E coli
Endoglucanase
Environmental impact
Enzymes
Ethanol
Fermented food
Glucan
Glycosidases
Glycosyl hydrolase
High temperature
Hydrolase
Lignocellulose
Molecular weight
Proteins
Reaction products
Sugar
Viscosity
β-Glucan
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Summary:In the context of avoiding the use of non-renewable energy sources, employing lignocellulosic biomass for ethanol production remains a challenge. Cellulases play an important role in this scenario: they are some of the most important industrial enzymes that can hydrolyze lignocellulose. This study aims to improve on the characterization of a thermostable endo-1,4-β-glucanase GH7 (Af-EGL7). To this end, Af-EGL7 was successfully expressed in X-33. The kinetic parameters and were estimated and suggested a robust enzyme. The recombinant protein was highly stable within an extreme pH range (3.0-8.0) and was highly thermostable at 55 °C for 72 h. Low Cu concentrations (0.1-1.0 mM) stimulated Af-EGL7 activity up to 117%. Af-EGL7 was tolerant to inhibition by products, such as glucose and cellobiose. Glucose at 50 mM did not inhibit Af-EGL7 activity, whereas 50 mM cellobiose inhibited Af-EGL7 activity by just 35%. Additionally, the Celluclast 1.5L cocktail supplemented with Af-EGL7 provided improved hydrolysis of sugarcane bagasse "in natura", sugarcane exploded bagasse (SEB), corncob, rice straw, and bean straw. In conclusion, the novel characterization of Af-EGL7 conducted in this study highlights the extraordinary properties that make Af-EGL7 a promising candidate for industrial applications.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20092261