Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins

Plant and microbial toxins are considered bioterrorism threat agents because of their extreme toxicity and/or ease of availability. Additionally, some of these toxins are increasingly responsible for accidental food poisonings. The current study utilized an ELISA-based protein antibody microarray fo...

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Bibliographic Details
Published in:Analyst (London) 2014-10, Vol.139 (20), p.5093-5102
Main Authors: Jenko, Kathryn L, Zhang, Yanfeng, Kostenko, Yulia, Fan, Yongfeng, Garcia-Rodriguez, Consuelo, Lou, Jianlong, Marks, James D, Varnum, Susan M
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies - immunology
Assaying
Bioterrorism
Botulinum Toxins - analysis
Buffers
Enterotoxins - analysis
Enzyme-Linked Immunosorbent Assay
Milk
Milk - chemistry
Multiplexing
Protein Array Analysis
Ricin - analysis
Shiga Toxins - analysis
Toxicity
Toxicology - methods
Toxins
Toxins, Biological - analysis
Toxins, Biological - blood
Toxins, Biological - urine
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Summary:Plant and microbial toxins are considered bioterrorism threat agents because of their extreme toxicity and/or ease of availability. Additionally, some of these toxins are increasingly responsible for accidental food poisonings. The current study utilized an ELISA-based protein antibody microarray for the multiplexed detection of ten biothreat toxins, botulinum neurotoxins (BoNT) A, B, C, D, E, F, ricin, shiga toxins 1 and 2 (Stx), and staphylococcus enterotoxin B (SEB), in buffer and complex biological matrices. The multiplexed assay displayed a sensitivity of 1.3 pg mL(-1) (BoNT/A, BoNT/B, SEB, Stx-1 and Stx-2), 3.3 pg mL(-1) (BoNT/C, BoNT/E, BoNT/F) and 8.2 pg mL(-1) (BoNT/D, ricin). All assays demonstrated high accuracy (75-120 percent recovery) and reproducibility (most coefficients of variation
ISSN:0003-2654
1364-5528
1364-5528
DOI:10.1039/c4an01270d