Sucrose supplementation suppressed the growth inhibition in polyhydroxyalkanoate-producing plants

Polyhydroxyalkanoate (PHA) is a thermoplastic polymer with several advantageous properties, including biomass origin, biocompatibility, and biodegradability. PHA is synthesized in transgenic plants harboring 3 enzymatic genes: phaA, phaB, and phaC (collectively referred to as phaABC). PHA-producing...

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Bibliographic Details
Published in:Plant Biotechnology 2017/03/31, Vol.34(1), pp.39-43
Main Authors: Yoshizumi, Takeshi, Yamada, Miwa, Higuchi-Takeuchi, Mieko, Matsumoto, Ken′ichiro, Taguchi, Seiichi, Matsui, Minami, Numata, Keiji
Format: Article
Language:English
Subjects:
Accumulation
Biocompatibility
Biodegradability
Biodegradation
Biomass
biosynthesis
Cotyledons
Cytosol
Cytotoxicity
Dwarfism
Enzymes
Inhibition
metabolic pathway
Metabolites
polyhydroxyalkanoate
Polyhydroxyalkanoates
Polyhydroxyalkanoic acid
Polymers
Recovery
Sucrose
Sugar
Supplements
Toxicity
Transgenic plants
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Summary:Polyhydroxyalkanoate (PHA) is a thermoplastic polymer with several advantageous properties, including biomass origin, biocompatibility, and biodegradability. PHA is synthesized in transgenic plants harboring 3 enzymatic genes: phaA, phaB, and phaC (collectively referred to as phaABC). PHA-producing plants exhibit severe growth inhibition that leads to extremely low PHA accumulation when these enzymes are localized in the cytosol. This growth inhibition could be attributed to the deleterious effects of the PHA biosynthetic pathway on endogenous essential metabolites or to PHA cytotoxicity itself. We performed precise morphological observations of phaABC-overexpressing Arabidopsis (ABC-ox), which displayed typical growth inhibition. On growth medium without sucrose, ABC-ox exhibited a pale green phenotype, dwarfism, including small cotyledons and true leaves, and short roots. ABC-ox partially recovered from this growth inhibition when the growth medium was supplemented with 1% sucrose. This recovery was reversed after ABC-ox grown on 1% sucrose medium was transferred to soil. ABC-ox grown on 1% sucrose medium not only demonstrated recovery from growth inhibition but were also the only examined plants with PHA accumulation, suggesting that growth inhibition was not caused by PHA cytotoxicity but rather by a lack of essential metabolites.
ISSN:1342-4580
1347-6114
DOI:10.5511/plantbiotechnology.16.1121a