Sampling duration and freezing temperature influence the analysed gastric inositol phosphate composition of pigs fed diets with different levels of phytase

• Published in: Animal Nutrition 2019-06, Vol.5 (2), p.196-201
• Main Authors: Laird, Steven, Kühn, Imke, Bedford, Michael R, Whitfield, Hayley, Miller, Helen M
• Format: Article
• Language: English
• Subjects: Short Communication
• ISSN: 2405-6545; 2405-6383
• DOI: 10.1016/j.aninu.2018.12.003

This experiment was conducted to determine the effects of time and freezing temperature during sampling on gastric phytate ( -inositol [MYO] hexakisphosphate [InsP ]), lower inositol phosphates (InsP ) and MYO concentrations in pigs fed diets containing different levels of phytase. Forty pigs were fed 1 of 4 wheat-barley diets on an basis for 28 d. The diets comprised a nutritionally adequate positive control (PC), a similar diet but with Ca and P reduced by 1.6 and 1.24 g/kg, respectively (NC), and the NC supplemented with 500 (NC + 500) or 2,000 (NC + 2000) FTU phytase/kg. At the end of the experiment, chyme were collected from the stomach, thoroughly mixed and 2 subsamples (30 mL) were frozen immediately: one snap-frozen at -79 °C and the other at -20 °C. The remaining chyme were left to sit at room temperature (20 °C) and further subsamples were collected and frozen as above at 5, 10 and 15 min from the point of mixing. There were linear reductions in gastric InsP concentration over time during sampling (