Splenic development and injury in premature lambs supported by the artificial placenta

The purpose of this study is to evaluate splenic effects during artificial placenta (AP) support. AP lambs (118–121 d, n = 14) were delivered and placed on the AP support for a goal of 10–14 days. Cannulation used right jugular drainage and umbilical vein reinfusion. Early (ETC; 115–120 d; n = 7) an...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pediatric surgery 2019-06, Vol.54 (6), p.1147-1152
Main Authors: McLeod, Jennifer S., Church, Joseph T., Coughlin, Megan A., Carr, Benjamin, Poling, Clinton, Sarosi, Ellery, Perkins, Elena M., Quinones, Matias Caceres, Hala, Pavel, Rabah, Raja, Freiheit, Elizabeth, Rojas-Pena, Alvaro, Bartlett, Robert H, Mychaliska, George B.
Format: Article
Language:English
Subjects:
Artificial placenta
Extracorporeal membrane oxygenation
Premature lambs
Premature spleen
Splenic development
Splenic injury
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c471t-4d064eb24a31b11a29b55d7d7c6cd9f81e39bb6dc61950f7b12beacb192348e43
cites cdi_FETCH-LOGICAL-c471t-4d064eb24a31b11a29b55d7d7c6cd9f81e39bb6dc61950f7b12beacb192348e43
container_end_page 1152
container_issue 6
container_start_page 1147
container_title Journal of pediatric surgery
container_volume 54
creator McLeod, Jennifer S.
Church, Joseph T.
Coughlin, Megan A.
Carr, Benjamin
Poling, Clinton
Sarosi, Ellery
Perkins, Elena M.
Quinones, Matias Caceres
Hala, Pavel
Rabah, Raja
Freiheit, Elizabeth
Rojas-Pena, Alvaro
Bartlett, Robert H
Mychaliska, George B.
description The purpose of this study is to evaluate splenic effects during artificial placenta (AP) support. AP lambs (118–121 d, n = 14) were delivered and placed on the AP support for a goal of 10–14 days. Cannulation used right jugular drainage and umbilical vein reinfusion. Early (ETC; 115–120 d; n = 7) and late (LTC; 125–131 d; n = 7) tissue controls were delivered and immediately sacrificed. Spleens were formalin fixed, H&E stained, and graded for injury, response to inflammation, and extramedullary hematopoiesis (EMH). CD68 and CD163 stains were used to assess for macrophage activation and density. Clinical variables were correlated with splenic scores. Groups were compared using Fisher's Exact Test and descriptive statistics. p 
doi_str_mv 10.1016/j.jpedsurg.2019.02.041
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6545267</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022346819301824</els_id><sourcerecordid>2196530292</sourcerecordid><originalsourceid>FETCH-LOGICAL-c471t-4d064eb24a31b11a29b55d7d7c6cd9f81e39bb6dc61950f7b12beacb192348e43</originalsourceid><addsrcrecordid>eNqFkU1rFEEQhhtRzGb1L4Q-eplJf8_ORZQQEyHgwcRr0x81SQ89H3bPLOy_t5dNgp48FVS99b5FPQhdUFJTQtVlX_cz-Lymx5oR2taE1UTQN2hDJaeVJLx5izaEMFZxoXZn6DznnpDSJvQ9OuOkJUzIZoN-_ZwjjMFhD3uI0zzAuGAzehzGfk2HUvCcYDDLmgBHM9iM8zrPU1rAY3vAyxNgk5bQBRdMxHM0rjiYD-hdZ2KGj891ix6-Xd9f3VZ3P26-X329q5xo6FIJT5QAy4Th1FJqWGul9I1vnHK-7XYUeGut8k7RVpKusZRZMM7SlnGxA8G36PPJd17tAP6YnUzUcwqDSQc9maD_nYzhST9Oe62kkEw1xeDTs0Gafq-QFz2E7CBGM8K0Zs1oqyQnrARukTpJXZpyTtC9xlCij1B0r1-g6CMUTZguUMrixd9Hvq69UCiCLycBlFftAySdXYDRgQ8J3KL9FP6X8QeZ2qQ7</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2196530292</pqid></control><display><type>article</type><title>Splenic development and injury in premature lambs supported by the artificial placenta</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>McLeod, Jennifer S. ; Church, Joseph T. ; Coughlin, Megan A. ; Carr, Benjamin ; Poling, Clinton ; Sarosi, Ellery ; Perkins, Elena M. ; Quinones, Matias Caceres ; Hala, Pavel ; Rabah, Raja ; Freiheit, Elizabeth ; Rojas-Pena, Alvaro ; Bartlett, Robert H ; Mychaliska, George B.</creator><creatorcontrib>McLeod, Jennifer S. ; Church, Joseph T. ; Coughlin, Megan A. ; Carr, Benjamin ; Poling, Clinton ; Sarosi, Ellery ; Perkins, Elena M. ; Quinones, Matias Caceres ; Hala, Pavel ; Rabah, Raja ; Freiheit, Elizabeth ; Rojas-Pena, Alvaro ; Bartlett, Robert H ; Mychaliska, George B.</creatorcontrib><description>The purpose of this study is to evaluate splenic effects during artificial placenta (AP) support. AP lambs (118–121 d, n = 14) were delivered and placed on the AP support for a goal of 10–14 days. Cannulation used right jugular drainage and umbilical vein reinfusion. Early (ETC; 115–120 d; n = 7) and late (LTC; 125–131 d; n = 7) tissue controls were delivered and immediately sacrificed. Spleens were formalin fixed, H&amp;E stained, and graded for injury, response to inflammation, and extramedullary hematopoiesis (EMH). CD68 and CD163 stains were used to assess for macrophage activation and density. Clinical variables were correlated with splenic scores. Groups were compared using Fisher's Exact Test and descriptive statistics. p &lt; 0.05 indicated significance. Mean survival for AP lambs was 12 ± 5 d. There was no necrosis found in any of the groups. Vascular congestion and sinusoidal histiocytosis did not significantly differ between AP and control groups (p = 0.72; p = 0.311). There were significantly more pigmented macrophages (p = 0.008), CD163 (p = &lt;0.001), and CD68 (p = &lt;0.001) stained cells in the AP group. ETC and LTC demonstrated more EMH than AP spleens (p = &lt;0.001). During AP support, spleens appear to develop normally and exhibit an appropriate inflammatory response. After initiation of AP support, EMH transitions away from the spleen. Research Paper/Therapeutic Potential. N/A.</description><identifier>ISSN: 0022-3468</identifier><identifier>EISSN: 1531-5037</identifier><identifier>DOI: 10.1016/j.jpedsurg.2019.02.041</identifier><identifier>PMID: 30902457</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Artificial placenta ; Extracorporeal membrane oxygenation ; Premature lambs ; Premature spleen ; Splenic development ; Splenic injury</subject><ispartof>Journal of pediatric surgery, 2019-06, Vol.54 (6), p.1147-1152</ispartof><rights>2019</rights><rights>Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-4d064eb24a31b11a29b55d7d7c6cd9f81e39bb6dc61950f7b12beacb192348e43</citedby><cites>FETCH-LOGICAL-c471t-4d064eb24a31b11a29b55d7d7c6cd9f81e39bb6dc61950f7b12beacb192348e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30902457$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McLeod, Jennifer S.</creatorcontrib><creatorcontrib>Church, Joseph T.</creatorcontrib><creatorcontrib>Coughlin, Megan A.</creatorcontrib><creatorcontrib>Carr, Benjamin</creatorcontrib><creatorcontrib>Poling, Clinton</creatorcontrib><creatorcontrib>Sarosi, Ellery</creatorcontrib><creatorcontrib>Perkins, Elena M.</creatorcontrib><creatorcontrib>Quinones, Matias Caceres</creatorcontrib><creatorcontrib>Hala, Pavel</creatorcontrib><creatorcontrib>Rabah, Raja</creatorcontrib><creatorcontrib>Freiheit, Elizabeth</creatorcontrib><creatorcontrib>Rojas-Pena, Alvaro</creatorcontrib><creatorcontrib>Bartlett, Robert H</creatorcontrib><creatorcontrib>Mychaliska, George B.</creatorcontrib><title>Splenic development and injury in premature lambs supported by the artificial placenta</title><title>Journal of pediatric surgery</title><addtitle>J Pediatr Surg</addtitle><description>The purpose of this study is to evaluate splenic effects during artificial placenta (AP) support. AP lambs (118–121 d, n = 14) were delivered and placed on the AP support for a goal of 10–14 days. Cannulation used right jugular drainage and umbilical vein reinfusion. Early (ETC; 115–120 d; n = 7) and late (LTC; 125–131 d; n = 7) tissue controls were delivered and immediately sacrificed. Spleens were formalin fixed, H&amp;E stained, and graded for injury, response to inflammation, and extramedullary hematopoiesis (EMH). CD68 and CD163 stains were used to assess for macrophage activation and density. Clinical variables were correlated with splenic scores. Groups were compared using Fisher's Exact Test and descriptive statistics. p &lt; 0.05 indicated significance. Mean survival for AP lambs was 12 ± 5 d. There was no necrosis found in any of the groups. Vascular congestion and sinusoidal histiocytosis did not significantly differ between AP and control groups (p = 0.72; p = 0.311). There were significantly more pigmented macrophages (p = 0.008), CD163 (p = &lt;0.001), and CD68 (p = &lt;0.001) stained cells in the AP group. ETC and LTC demonstrated more EMH than AP spleens (p = &lt;0.001). During AP support, spleens appear to develop normally and exhibit an appropriate inflammatory response. After initiation of AP support, EMH transitions away from the spleen. Research Paper/Therapeutic Potential. N/A.</description><subject>Artificial placenta</subject><subject>Extracorporeal membrane oxygenation</subject><subject>Premature lambs</subject><subject>Premature spleen</subject><subject>Splenic development</subject><subject>Splenic injury</subject><issn>0022-3468</issn><issn>1531-5037</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFkU1rFEEQhhtRzGb1L4Q-eplJf8_ORZQQEyHgwcRr0x81SQ89H3bPLOy_t5dNgp48FVS99b5FPQhdUFJTQtVlX_cz-Lymx5oR2taE1UTQN2hDJaeVJLx5izaEMFZxoXZn6DznnpDSJvQ9OuOkJUzIZoN-_ZwjjMFhD3uI0zzAuGAzehzGfk2HUvCcYDDLmgBHM9iM8zrPU1rAY3vAyxNgk5bQBRdMxHM0rjiYD-hdZ2KGj891ix6-Xd9f3VZ3P26-X329q5xo6FIJT5QAy4Th1FJqWGul9I1vnHK-7XYUeGut8k7RVpKusZRZMM7SlnGxA8G36PPJd17tAP6YnUzUcwqDSQc9maD_nYzhST9Oe62kkEw1xeDTs0Gafq-QFz2E7CBGM8K0Zs1oqyQnrARukTpJXZpyTtC9xlCij1B0r1-g6CMUTZguUMrixd9Hvq69UCiCLycBlFftAySdXYDRgQ8J3KL9FP6X8QeZ2qQ7</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>McLeod, Jennifer S.</creator><creator>Church, Joseph T.</creator><creator>Coughlin, Megan A.</creator><creator>Carr, Benjamin</creator><creator>Poling, Clinton</creator><creator>Sarosi, Ellery</creator><creator>Perkins, Elena M.</creator><creator>Quinones, Matias Caceres</creator><creator>Hala, Pavel</creator><creator>Rabah, Raja</creator><creator>Freiheit, Elizabeth</creator><creator>Rojas-Pena, Alvaro</creator><creator>Bartlett, Robert H</creator><creator>Mychaliska, George B.</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20190601</creationdate><title>Splenic development and injury in premature lambs supported by the artificial placenta</title><author>McLeod, Jennifer S. ; Church, Joseph T. ; Coughlin, Megan A. ; Carr, Benjamin ; Poling, Clinton ; Sarosi, Ellery ; Perkins, Elena M. ; Quinones, Matias Caceres ; Hala, Pavel ; Rabah, Raja ; Freiheit, Elizabeth ; Rojas-Pena, Alvaro ; Bartlett, Robert H ; Mychaliska, George B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-4d064eb24a31b11a29b55d7d7c6cd9f81e39bb6dc61950f7b12beacb192348e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Artificial placenta</topic><topic>Extracorporeal membrane oxygenation</topic><topic>Premature lambs</topic><topic>Premature spleen</topic><topic>Splenic development</topic><topic>Splenic injury</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McLeod, Jennifer S.</creatorcontrib><creatorcontrib>Church, Joseph T.</creatorcontrib><creatorcontrib>Coughlin, Megan A.</creatorcontrib><creatorcontrib>Carr, Benjamin</creatorcontrib><creatorcontrib>Poling, Clinton</creatorcontrib><creatorcontrib>Sarosi, Ellery</creatorcontrib><creatorcontrib>Perkins, Elena M.</creatorcontrib><creatorcontrib>Quinones, Matias Caceres</creatorcontrib><creatorcontrib>Hala, Pavel</creatorcontrib><creatorcontrib>Rabah, Raja</creatorcontrib><creatorcontrib>Freiheit, Elizabeth</creatorcontrib><creatorcontrib>Rojas-Pena, Alvaro</creatorcontrib><creatorcontrib>Bartlett, Robert H</creatorcontrib><creatorcontrib>Mychaliska, George B.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of pediatric surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McLeod, Jennifer S.</au><au>Church, Joseph T.</au><au>Coughlin, Megan A.</au><au>Carr, Benjamin</au><au>Poling, Clinton</au><au>Sarosi, Ellery</au><au>Perkins, Elena M.</au><au>Quinones, Matias Caceres</au><au>Hala, Pavel</au><au>Rabah, Raja</au><au>Freiheit, Elizabeth</au><au>Rojas-Pena, Alvaro</au><au>Bartlett, Robert H</au><au>Mychaliska, George B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Splenic development and injury in premature lambs supported by the artificial placenta</atitle><jtitle>Journal of pediatric surgery</jtitle><addtitle>J Pediatr Surg</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>54</volume><issue>6</issue><spage>1147</spage><epage>1152</epage><pages>1147-1152</pages><issn>0022-3468</issn><eissn>1531-5037</eissn><abstract>The purpose of this study is to evaluate splenic effects during artificial placenta (AP) support. AP lambs (118–121 d, n = 14) were delivered and placed on the AP support for a goal of 10–14 days. Cannulation used right jugular drainage and umbilical vein reinfusion. Early (ETC; 115–120 d; n = 7) and late (LTC; 125–131 d; n = 7) tissue controls were delivered and immediately sacrificed. Spleens were formalin fixed, H&amp;E stained, and graded for injury, response to inflammation, and extramedullary hematopoiesis (EMH). CD68 and CD163 stains were used to assess for macrophage activation and density. Clinical variables were correlated with splenic scores. Groups were compared using Fisher's Exact Test and descriptive statistics. p &lt; 0.05 indicated significance. Mean survival for AP lambs was 12 ± 5 d. There was no necrosis found in any of the groups. Vascular congestion and sinusoidal histiocytosis did not significantly differ between AP and control groups (p = 0.72; p = 0.311). There were significantly more pigmented macrophages (p = 0.008), CD163 (p = &lt;0.001), and CD68 (p = &lt;0.001) stained cells in the AP group. ETC and LTC demonstrated more EMH than AP spleens (p = &lt;0.001). During AP support, spleens appear to develop normally and exhibit an appropriate inflammatory response. After initiation of AP support, EMH transitions away from the spleen. Research Paper/Therapeutic Potential. N/A.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>30902457</pmid><doi>10.1016/j.jpedsurg.2019.02.041</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0022-3468
ispartof Journal of pediatric surgery, 2019-06, Vol.54 (6), p.1147-1152
issn 0022-3468
1531-5037
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6545267
source ScienceDirect Freedom Collection 2022-2024
subjects Artificial placenta
Extracorporeal membrane oxygenation
Premature lambs
Premature spleen
Splenic development
Splenic injury
title Splenic development and injury in premature lambs supported by the artificial placenta
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T09%3A27%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Splenic%20development%20and%20injury%20in%20premature%20lambs%20supported%20by%20the%20artificial%20placenta&rft.jtitle=Journal%20of%20pediatric%20surgery&rft.au=McLeod,%20Jennifer%20S.&rft.date=2019-06-01&rft.volume=54&rft.issue=6&rft.spage=1147&rft.epage=1152&rft.pages=1147-1152&rft.issn=0022-3468&rft.eissn=1531-5037&rft_id=info:doi/10.1016/j.jpedsurg.2019.02.041&rft_dat=%3Cproquest_pubme%3E2196530292%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c471t-4d064eb24a31b11a29b55d7d7c6cd9f81e39bb6dc61950f7b12beacb192348e43%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2196530292&rft_id=info:pmid/30902457&rfr_iscdi=true