Chlamydia trachomatis samples testing falsely negative in the Aptima Combo 2 test in Finland, 2019

Since February 2019, over 160 (CT) cases testing negative or equivocal by Aptima Combo 2 (AC2) but positive by Aptima CT test run with Panther instruments occurred in Finland. The AC2 test targets chlamydial 23S rRNA while the CT test targets 16S rRNA. Sequencing of 10 strains revealed a nucleotide...

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Bibliographic Details
Published in:Euro surveillance : bulletin européen sur les maladies transmissibles 2019-05, Vol.24 (22), p.1
Main Authors: Rantakokko-Jalava, Kaisu, Hokynar, Kati, Hieta, Niina, Keskitalo, Anniina, Jokela, Pia, Muotiala, Anna, Jokiranta, T Sakari, Kuusela, Rutta, Sarkkinen, Hannu, Aittoniemi, Janne, Vuorinen, Tytti, Hakanen, Antti J, Puolakkainen, Mirja
Format: Article
Language:English
Subjects:
Adolescent
Adult
Bacteriological Techniques - methods
Bacteriological Techniques - standards
Chlamydia
Chlamydia Infections - diagnosis
Chlamydia Infections - epidemiology
Chlamydia Infections - genetics
Chlamydia trachomatis - genetics
Chlamydia trachomatis - isolation & purification
Diagnostic tests
False Negative Reactions
Female
Finland - epidemiology
Humans
Immunoassay
Male
Medical errors
Middle Aged
Rapid Communication
Reagent Kits, Diagnostic - standards
Young Adult
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Summary:Since February 2019, over 160 (CT) cases testing negative or equivocal by Aptima Combo 2 (AC2) but positive by Aptima CT test run with Panther instruments occurred in Finland. The AC2 test targets chlamydial 23S rRNA while the CT test targets 16S rRNA. Sequencing of 10 strains revealed a nucleotide substitution in 23S rRNA. The significance of this for the failure of the AC2 test to detect the variant is not yet known.
ISSN:1560-7917
1025-496X
1560-7917
DOI:10.2807/1560-7917.ES.2019.24.22.1900298